Transcription factors (TFs) are key regulators of intrinsic cellular processes, such as
differentiation and development, and of the cellular response to external perturbation …

Inevitable byproducts of aerobic metabolism are free radicals and other highly reactive
oxidants and nitrosants that are continuously formed within cells. Most cells generally …

Posttranslational modifications (PTM s) of proteins greatly expand proteome diversity,
increase functionality, and allow for rapid responses, all at relatively low costs for the cell …

Access to high-quality antibodies is a necessity for the study of histones and their
posttranslational modifications (PTMs). Here we debut the Histone Antibody Specificity …

Post-translational modifications (PTMs) have been recognized to impact protein function in
two ways:(i) orthosterically, via direct recognition by protein domains or through interference …

Histone post-translational modifications (PTMs) comprise one of the most intricate nuclear
signaling networks that govern gene expression in a long-term and dynamic fashion. These …

MS analysis of intact protein complexes has emerged as an established technology for
assessing the composition and connectivity within dynamic, heterogeneous multiprotein …

Enrichment, separation and mass spectrometric analysis of biomolecules carrying a
phosphate group plays an important role in current analytical chemistry. Application areas …

We outline NMR protocols for site-specific mapping and time-resolved monitoring of protein
phosphorylation reactions using purified kinases and mammalian cell extracts. These …

Histones and their posttranslational modifications (PTMs) play an important role in
regulating DNA-templated processes. While some PTMs directly modulate chromatin …