Sequencing of the 7 kb immC region from four P1-related phages identified a novel DNA
recombinase that exhibits many Cre-like characteristics, including recombination in …

With the completion of Sc2. 0 chromosomes, synthetic chromosome rearrangement and
modification by loxP-mediated evolution (SCRaMbLE) becomes more critical for in-depth …

Recombinases have several potential advantages as genome editing tools compared to
nucleases and other editing enzymes, but the process of engineering them to efficiently …

Currently available inducible Cre/loxP systems, despite their considerable utility in gene
manipulation, have pitfalls in certain scenarios, such as unsatisfactory recombination rates …

The invention provides a Dre/rox recombinase system. In particular, the invention provides
Dre polypeptides that can catalyze site-specific recombination at roX sites but not at loX …

Tyrosine site-specific recombinases (SSRs) represent a versatile genome editing tool with
considerable therapeutic potential. Recent developments to engineer and evolve SSRs into …

The 16 kDa cysteine-rich protein (16K) of tobacco rattle virus (TRV) is known to partially
suppress RNA silencing in Drosophila cells. In this study, we show that 16K suppresses …

During mitosis, kinetochores physically link chromosomes to the dynamic ends of spindle
microtubules. This linkage depends on the Ndc80 complex, a conserved and essential …

Current approaches to protein site-directed mutagenesis require an independent user
operation for each mutation. This can impede large-scale scanning mutagenesis projects …

Engineering of alternative binding sites on the surface of an enzyme while preserving the
enzymatic activity would offer new opportunities for controlling the activity by binding of non …