H Zhang, X Fu, X Gong, Y Wang, H Zhang… - Nature …, 2022 - nature.com
With the completion of Sc2. 0 chromosomes, synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE) becomes more critical for in-depth …
Recombinases have several potential advantages as genome editing tools compared to nucleases and other editing enzymes, but the process of engineering them to efficiently …
F Meng, Y Han, D Srisai, V Belakhov… - Proceedings of the …, 2016 - National Acad Sciences
Currently available inducible Cre/loxP systems, despite their considerable utility in gene manipulation, have pitfalls in certain scenarios, such as unsatisfactory recombination rates …
BL Sauer, J McDermott - US Patent 7,422,889, 2008 - Google Patents
The invention provides a Dre/rox recombinase system. In particular, the invention provides Dre polypeptides that can catalyze site-specific recombination at roX sites but not at loX …
J Hoersten, G Ruiz-Gómez, F Lansing… - Nucleic Acids …, 2022 - academic.oup.com
Tyrosine site-specific recombinases (SSRs) represent a versatile genome editing tool with considerable therapeutic potential. Recent developments to engineer and evolve SSRs into …
W Ghazala, A Waltermann, R Pilot… - Journal of General …, 2008 - microbiologyresearch.org
The 16 kDa cysteine-rich protein (16K) of tobacco rattle virus (TRV) is known to partially suppress RNA silencing in Drosophila cells. In this study, we show that 16K suppresses …
During mitosis, kinetochores physically link chromosomes to the dynamic ends of spindle microtubules. This linkage depends on the Ndc80 complex, a conserved and essential …
KA Daggett, M Layer, TA Cropp - ACS chemical biology, 2009 - ACS Publications
Current approaches to protein site-directed mutagenesis require an independent user operation for each mutation. This can impede large-scale scanning mutagenesis projects …
P Mathonet, J Deherve, P Soumillion… - Protein science, 2006 - Wiley Online Library
Engineering of alternative binding sites on the surface of an enzyme while preserving the enzymatic activity would offer new opportunities for controlling the activity by binding of non …