Optimizing VLP production in gene therapy: Opportunities and challenges for in silico modeling

L Zehetner, D Széliová, B Kraus… - Biotechnology …, 2023 - Wiley Online Library
Over the past decades, virus‐like particle (VLP)‐based gene therapy (GT) evolved as a
promising approach to cure inherited diseases or cancer. Tremendous costs due to …

State-of-the-art approaches to heterologous expression of bispecific antibodies targeting solid tumors

AK Misorin, DO Chernyshova, MS Karbyshev - Biochemistry (Moscow), 2023 - Springer
Bispecific antibodies (bsAbs) are some of the most promising biotherapeutics due to the
versatility provided by their structure and functional features. bsAbs simultaneously bind two …

Generation of a highly specific recombinant full-length antibody for detecting ethirimol in fruit and environmental water

X Lei, P Li, AM Abd El-Aty, J Zhao, L Xu, S Gao… - Journal of Hazardous …, 2024 - Elsevier
High-performance antibodies are core reagents for highly sensitive immunoassays. Herein,
based on a novel hapten, a hybridoma secreting the high-affinity anti-ethirimol monoclonal …

Long-Term Culturing of FreeStyle 293-F Cells Affects Immunoglobulin G Glycome Composition

F Lukšić, A Mijakovac, G Josipović, V Vičić Bočkor… - Biomolecules, 2023 - mdpi.com
Glycosylation of IgG regulates the effector function of this antibody in the immune response.
Glycosylated IgG is a potent therapeutic used for both research and clinical purposes. While …

Exploring Parametric and Mechanistic Differences between Expi293FTM and ExpiCHO-STM Cells for Transient Antibody Production Optimization

J Zhou, GG Yan, D Cluckey, C Meade, M Ruth, R Sorm… - Antibodies, 2023 - mdpi.com
Rapidly producing drug-like antibody therapeutics for lead molecule discovery and
candidate optimization is typically accomplished by large-scale transient gene expression …

[HTML][HTML] Deciphering molecular drivers of lactate metabolic shift in mammalian cell cultures

M Torres, E Hawke, R Hoare, R Scholey, LP Pybus… - Metabolic …, 2025 - Elsevier
Lactate metabolism plays a critical role in mammalian cell bioprocessing, influencing
cellular performance and productivity. The transition from lactate production to consumption …

Protein production from HEK293 cell line-derived stable pools with high protein quality and quantity to support discovery research

H Sun, S Wang, M Lu, CE Tinberg, BM Alba - PLoS One, 2023 - journals.plos.org
Antibody-based therapeutics and recombinant protein reagents are often produced in
mammalian expression systems, which provide human-like post-translational modifications …

HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing

SGR Gurazada, HM Kennedy, RD Braatz… - Biotechnology …, 2024 - Elsevier
Gene therapy is poised to transition from niche to mainstream medicine, with recombinant
adeno-associated virus (rAAV) as the vector of choice. However, robust, scalable …

Recent strategies to achieve high production yield of recombinant protein: A review

CZ Tepap, J Anissi, S Bounou - Journal of Cellular …, 2023 - content.iospress.com
Recombinant proteins have long been used in the pharmaceutical, chemical, and
agricultural industries. These proteins can be produced in hosts such as mammalian cells …

Design and optimization of an IgG human ELISA assay reactive to recombinant RBD SARS-CoV-2 protein

MC Rodríguez, N Ceaglio, A Gugliotta… - Applied Microbiology …, 2022 - Springer
Serology assays are essential tools to mitigate the effect of COVID-19, help to identify
previous SARS-CoV-2 infections or vaccination, and provide data for surveillance and …