Identifying cell states during development from their mRNA profiles provides insight into their
gene regulatory network. Here, we leverage the sea urchin embryo for its well-established …

The germline is unique and immortal (or at least its genome is). It is able to perform unique
jobs (meiosis) and is selected for genetic changes. Part of being this special also means that …

Altered regulatory interactions during development likely underlie a large fraction of
phenotypic diversity within and between species, yet identifying specific evolutionary …

Echinoderms represent a broad phylum with many tractable features to test evolutionary
changes and constraints. Here, we present a single-cell RNA-sequencing analysis of early …

We seek to manipulate gene function here through CRISPR-Cas9 editing of cis-regulatory
sequences, rather than the more typical mutation of coding regions. This approach would …

Primordial germ cells (PGCs) are specified during development by either one of two major
mechanisms, the preformation mode or the inductive mode. Because the inductive mode is …

Evolutionary transitions are particularly important in development of the germ line, cells
which directly impact sexual reproduction. Differences in the primordial germ cells (PGCs) of …

Echinoderms are a major model system for many general aspects of biology, including
mechanisms of gene regulation. Analysis of transcriptional regulation (Gene regulatory …

The production of germ cells, especially primordial germ cells (PGCs), is important for avian
stem cells and reproduction biology. However, key factors involved in the regulation of PGCs …

Abstract Specification of primordial germ cells (PGCs), the lineage which gives rise to eggs
and sperm, is essential for sexually reproducing organisms. The mechanism by which …