VIGS goes viral: how VIGS transforms our understanding of plant science

C Rössner, D Lotz, A Becker - Annual Review of Plant Biology, 2022 - annualreviews.org
Virus-induced gene silencing (VIGS) has developed into an indispensable approach to
gene function analysis in a wide array of species, many of which are not amenable to stable …

Virus-induced gene silencing (VIGS): a powerful tool for crop improvement and its advancement towards epigenetics

S Zulfiqar, MA Farooq, T Zhao, PP Wang… - International journal of …, 2023 - mdpi.com
Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technology that
has evolved into an indispensable approach for analyzing the function of genes. It …

Efficient and high-throughput pseudorecombinant-chimeric Cucumber mosaic virus-based VIGS in maize

H Li, D Zhang, K Xie, Y Wang, Q Liao, Y Hong… - Plant …, 2021 - academic.oup.com
Virus-induced gene silencing (VIGS) is a versatile and attractive approach for functional
gene characterization in plants. Although several VIGS vectors for maize (Zea mays) have …

Genome-wide analysis of ribosomal protein GhRPS6 and its role in cotton Verticillium wilt resistance

D Zhu, X Zhang, J Zhou, Y Wu, X Zhang… - International journal of …, 2021 - mdpi.com
Verticillium wilt is threatening the world's cotton production. The pathogenic fungus
Verticillium dahliae can survive in the soil in the form of microsclerotia for a long time …

Plant biomacromolecule delivery methods in the 21st century

S Rustgi, S Naveed, J Windham, H Zhang… - Frontiers in Genome …, 2022 - frontiersin.org
The 21st century witnessed a boom in plant genomics and gene characterization studies
through RNA interference and site-directed mutagenesis. Specifically, the last 15 years …

Unraveling the functional characterization of a jasmonate-induced flavonoid biosynthetic CYP45082G24 gene in Carthamus tinctorius

Y Wang, Z Li, N Ahmad, X Sheng, B Iqbal… - Functional & Integrative …, 2023 - Springer
The cytochrome P450 superfamily of monooxygenases plays a major role in the evolution
and diversification of plant natural products. The function of cytochrome P450s in …

Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies

R Sharma, Y Liang, MY Lee, VR Pidatala… - BMC research …, 2020 - Springer
Objectives Sorghum is one of the most recalcitrant species for transformation. Considering
the time and effort required for stable transformation in sorghum, establishing a transient …

Rescue of the first alphanucleorhabdovirus entirely from cloned complementary DNA: an efficient vector for systemic expression of foreign genes in maize and insect …

S Kanakala, CAD Xavier, KM Martin… - Molecular Plant …, 2023 - Wiley Online Library
Recent reverse genetics technologies have enabled genetic manipulation of plant negative‐
strand RNA virus (NSR) genomes. Here, we report construction of an infectious clone for the …

SIGS vs. HIGS: Opportunities and challenges of RNAi pest and pathogen control strategies

BM Beernink, N Amanat, VH Li… - Canadian Journal of …, 2024 - Taylor & Francis
Insect pests and fungal pathogens are estimated to cause 20–40% yield reduction to crops
annually, causing $290 billion of economic loss every season worldwide. Pest and …

Foxtail mosaic virus: A tool for gene function analysis in maize and other monocots

BM Beernink, SA Whitham - Molecular Plant Pathology, 2023 - Wiley Online Library
Many plant viruses have been engineered into vectors for use in functional genomics
studies, expression of heterologous proteins, and, most recently, gene editing applications …