TALE nucleases: tailored genome engineering made easy
C Mussolino, T Cathomen - Current opinion in biotechnology, 2012 - Elsevier
Custom-made designer nucleases have evolved into an indispensable platform to precisely
alter complex genomes for basic research, biotechnology, synthetic biology, or human gene …
alter complex genomes for basic research, biotechnology, synthetic biology, or human gene …
Zinc-finger nuclease based genome surgery: it's all about specificity
EM Handel, T Cathomen - Current gene therapy, 2011 - ingentaconnect.com
Therapeutic genome engineering is a hallmark of gene therapy but only recent
technological advances have permitted the modification of complex genomes in a targeted …
technological advances have permitted the modification of complex genomes in a targeted …
Template plasmid integration in germline genome-edited cattle
AL Norris, SS Lee, KJ Greenlees, DA Tadesse… - Nature …, 2020 - nature.com
16526–16531 (2013). 9. Medugorac, I. et al. Bovine polledness—an autosomal dominant
trait with allelic heterogeneity. PLoS ONE 7, e39477 (2012). 10. Olsen, PA, Gelazauskaite …
trait with allelic heterogeneity. PLoS ONE 7, e39477 (2012). 10. Olsen, PA, Gelazauskaite …
High-frequency random DNA insertions upon co-delivery of CRISPR-Cas9 ribonucleoprotein and selectable marker plasmid in rice
An important advantage of delivering CRISPR reagents into cells as a ribonucleoprotein
(RNP) complex is the ability to edit genes without reagents being integrated into the …
(RNP) complex is the ability to edit genes without reagents being integrated into the …
Design and specificity of long ssDNA donors for CRISPR-based knock-in
CRISPR/Cas technologies have transformed our ability to manipulate genomes for research
and gene-based therapy. In particular, homology-directed repair after genomic cleavage …
and gene-based therapy. In particular, homology-directed repair after genomic cleavage …
Transcription activator like effector (TALE)-directed piggyBac transposition in human cells
JB Owens, D Mauro, I Stoytchev, MS Bhakta… - Nucleic acids …, 2013 - academic.oup.com
Insertional therapies have shown great potential for combating genetic disease and safer
methods would undoubtedly broaden the variety of possible illness that can be treated. A …
methods would undoubtedly broaden the variety of possible illness that can be treated. A …
Zinc-finger nucleases for somatic gene therapy: the next frontier
SH Rahman, ML Maeder, JK Joung… - Human gene …, 2011 - liebertpub.com
Zinc-finger nucleases (ZFNs) are a powerful tool that can be used to edit the human genome
ad libitum. The technology has experienced remarkable development in the last few years …
ad libitum. The technology has experienced remarkable development in the last few years …
DNA nicks promote efficient and safe targeted gene correction
L Davis, N Maizels - PloS one, 2011 - journals.plos.org
Targeted gene correction employs a site-specific DNA lesion to promote homologous
recombination that eliminates mutation in a disease gene of interest. The double-strand …
recombination that eliminates mutation in a disease gene of interest. The double-strand …
CCR5 gene editing of resting CD4+ T cells by transient ZFN expression from HIV envelope pseudotyped nonintegrating lentivirus confers HIV-1 resistance in …
CCR5 disruption by zinc finger nucleases (ZFNs) is a promising method for HIV-1 gene
therapy. However, successful clinical translation of this strategy necessitates the …
therapy. However, successful clinical translation of this strategy necessitates the …
Human germline genetic modification: scientific and bioethical perspectives
KR Smith, S Chan, J Harris - Archives of medical research, 2012 - Elsevier
The latest mammalian genetic modification technology offers efficient and reliable targeting
of genomic sequences, in the guise of designer genetic recombination tools. These and …
of genomic sequences, in the guise of designer genetic recombination tools. These and …