Closing the door with CRISPR: genome editing of CCR5 and CXCR4 as a potential curative solution for HIV

JJ Freen-van Heeren - BioTech, 2022 - mdpi.com
Human immunodeficiency virus (HIV) infection can be controlled by anti-retroviral therapy.
Suppressing viral replication relies on life-long medication, but anti-retroviral therapy is not …

Flow-FISH as a tool for studying bacteria, fungi and viruses

JJ Freen-van Heeren - BioTech, 2021 - mdpi.com
Many techniques are currently in use to study microbes. These can be aimed at detecting,
identifying, and characterizing bacterial, fungal, and viral species. One technique that is …

Costimulation through TLR2 drives polyfunctional CD8+ T cell responses

F Salerno, JJ Freen-van Heeren, A Guislain… - The Journal of …, 2019 - journals.aai.org
Optimal T cell activation requires Ag recognition through the TCR, engagement of
costimulatory molecules, and cytokines. T cells can also directly recognize danger signals …

Expression of IL-37 induces a regulatory T-cell-like phenotype and function in Jurkat cells

DG Osborne, J Domenico, M Fujita - Cells, 2022 - mdpi.com
The anti-inflammatory cytokine interleukin-37 (IL-37) plays a key role in inhibiting innate and
adaptive immunity. Past results have shown that IL-37 is elevated in human Treg cells …

Toll‐like receptor‐2/7‐mediated T cell activation: An innate potential to augment CD8+ T cell cytokine production

JJ Freen‐van Heeren - Scandinavian journal of immunology, 2021 - Wiley Online Library
CD8+ T cells are critical to combat pathogens and eradicate malignantly transformed cells.
To exert their effector function and kill target cells, T cells produce copious amounts of …

Combined single-cell measurement of cytokine mRNA and protein in immune cells

JJ Freen-van Heeren, BP Nicolet… - Immune Mediators in …, 2020 - Springer
A key feature of immune cells, such as T cells, is their rapid responsiveness to activation.
The response rate of T cells depends on the signal strength, and the type of signals they …

Establishment of a rapid counting method for lactic acid bacteria and yeast in dairy products

Y Li, C Wang, J Wang - International Journal of Dairy …, 2024 - Wiley Online Library
The plate count agar (PCA) method is a common technique used to count microorganisms in
dairy products, but this method is time‐consuming and highly selective by the medium. This …

Post‐transcriptional control of T‐cell cytokine production: Implications for cancer therapy

JJ Freen‐van Heeren - Immunology, 2021 - Wiley Online Library
As part of the adaptive immune system, T cells are vital for the eradication of infected and
malignantly transformed cells. To perform their protective function, T cells produce effector …

[HTML][HTML] FISHing for COVID‐19: Studying SARS‐CoV‐2 with single‐molecule FISH and flow‐FISH

JJ Freen‐van Heeren - Cytometry, 2022 - ncbi.nlm.nih.gov
For more than 2 years, the COVID-19 pandemic has plagued the world. The causative
agent, SARS-CoV-2, is a single-stranded RNA virus that is easily transmitted, resulting in> …

Assessing Antigen-Specific T Cell Responses Through IFN-γ Enzyme-Linked Immune Absorbent Spot (ELISpot)

JJ Freen-van Heeren, V Palomares Cabeza… - … : Methods and Protocols, 2024 - Springer
T cells are instrumental in protecting the host against invading pathogens and the
development of cancer. To do so, they produce effector molecules such as granzymes …