Ensiling of forages was recognized as a microbial-driven process as early as the late 1800s,
when it was associated with the production of “sweet” or “sour” silage. Classical …

Background Microbiome studies often involve sequencing a marker gene to identify the
microorganisms in samples of interest. Sequence classification is a critical component of this …

Microbial community analysis using 16S rRNA gene amplicon sequencing is the backbone
of many microbial ecology studies. Several approaches and pipelines exist for processing …

Thanks to the tremendous advancement of deep sequencing and large-scale profiling,
epitranscriptomics has become a rapidly growing field. As one of the most important parts of …

Background Pseudouridylation is the most prevalent type of posttranscriptional modification
in various stable RNAs of all organisms, which significantly affects many cellular processes …

Background The low cost of 16S rRNA gene sequencing facilitates population-scale
molecular epidemiological studies. Existing computational algorithms can resolve 16S rRNA …

Insects account for a large portion of Earth's biodiversity and are key players for ecosystems,
notably as pollinators. While insect migration is suspected to represent a natural …

Next generation sequencing technologies have vastly changed the approach of sequencing
of the 16S rRNA gene for studies in microbial ecology. Three distinct technologies are …

As one of the most ubiquitous post-transcriptional modifications of RNA, N 6-
methyladenosine (m 6 A) plays an essential role in many vital biological processes. The …

Background Reads assignment to taxonomic units is a key step in microbiome analysis
pipelines. To date, accurate taxonomy annotation of 16S reads, particularly at species rank …