The sound of silence: Transgene silencing in mammalian cell engineering
A Cabrera, HI Edelstein, F Glykofrydis, KS Love… - Cell Systems, 2022 - cell.com
To elucidate principles operating in native biological systems and to develop novel
biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within …
biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within …
[HTML][HTML] Decoding enhancers using massively parallel reporter assays
Enhancers control the timing, location and expression levels of their target genes.
Nucleotide variation in enhancers has been shown to lead to numerous phenotypes …
Nucleotide variation in enhancers has been shown to lead to numerous phenotypes …
A systematic comparison reveals substantial differences in chromosomal versus episomal encoding of enhancer activity
Candidate enhancers can be identified on the basis of chromatin modifications, the binding
of chromatin modifiers and transcription factors and cofactors, or chromatin accessibility …
of chromatin modifiers and transcription factors and cofactors, or chromatin accessibility …
Assessing sufficiency and necessity of enhancer activities for gene expression and the mechanisms of transcription activation
RR Catarino, A Stark - Genes & development, 2018 - genesdev.cshlp.org
Enhancers are important genomic regulatory elements directing cell type-specific
transcription. They assume a key role during development and disease, and their …
transcription. They assume a key role during development and disease, and their …
Functional dissection of the enhancer repertoire in human embryonic stem cells
Enhancers are genetic elements that regulate spatiotemporal gene expression. Enhancer
function requires transcription factor (TF) binding and correlates with histone modifications …
function requires transcription factor (TF) binding and correlates with histone modifications …
GATA transcription factors drive initial Xist upregulation after fertilization through direct activation of long-range enhancers
L Ravid Lustig, A Sampath Kumar, T Schwämmle… - Nature Cell …, 2023 - nature.com
X-chromosome inactivation (XCI) balances gene expression between the sexes in female
mammals. Shortly after fertilization, upregulation of Xist RNA from one X chromosome …
mammals. Shortly after fertilization, upregulation of Xist RNA from one X chromosome …
In pursuit of design principles of regulatory sequences
Instructions for when, where and to what level each gene should be expressed are encoded
within regulatory sequences. The importance of motifs recognized by DNA-binding …
within regulatory sequences. The importance of motifs recognized by DNA-binding …
Enhancer logic and mechanics in development and disease
R Rickels, A Shilatifard - Trends in cell biology, 2018 - cell.com
Enhancers are distally located genomic cis-regulatory elements that integrate
spatiotemporal cues to coordinate gene expression in a tissue-specific manner during …
spatiotemporal cues to coordinate gene expression in a tissue-specific manner during …
Enhancers and super-enhancers have an equivalent regulatory role in embryonic stem cells through regulation of single or multiple genes
Transcriptional enhancers are critical for maintaining cell-type–specific gene expression and
driving cell fate changes during development. Highly transcribed genes are often associated …
driving cell fate changes during development. Highly transcribed genes are often associated …
Gene regulatory elements, major drivers of human disease
S Chatterjee, N Ahituv - Annual review of genomics and human …, 2017 - annualreviews.org
Gene expression changes, the driving forces for cellular diversity in multicellular organisms,
are regulated by a diverse set of gene regulatory elements that direct transcription in specific …
are regulated by a diverse set of gene regulatory elements that direct transcription in specific …