Ribonucleases (RNases) are mediators in most reactions of RNA metabolism. In recent
years, there has been a surge of new information about RNases and the roles they play in …

mRNA degradation is an important mechanism for controlling gene expression in bacterial
cells. This process involves the orderly action of a battery of cellular endonucleases and …

Structural biology studies performed inside cells can capture molecular machines in action
within their native context. In this work, we developed an integrative in-cell structural …

Even though Bacillus subtilis is one of the most studied organisms, no function has been
identified for about 20% of its proteins. Among these unknown proteins are several RNA-and …

Small non-coding RNAs (sRNAs) are critical post-transcriptional regulators of gene
expression. Distinct RNA-binding proteins (RBPs) influence the processing, stability and …

Bacteria have a remarkable ability to sense environmental changes, swiftly regulating their
transcriptional and posttranscriptional machinery as a response. Under conditions that …

Single-molecule (particle) tracking is a powerful method to study dynamic processes in cells
at highest possible spatial and temporal resolution. We have developed SMTracker, a …

The instability of messenger RNA is fundamental to the control of gene expression. In
bacteria, mRNA degradation generally follows an “all-or-none” pattern. This implies that if …

RNA turnover plays an important role in both virulence and adaptation to stress in the Gram-
positive human pathogen Staphylococcus aureus. However, the molecular players and …

Highlights•Bacterial RNases control sRNA-mediated negative and positive
regulation.•RNase III can simultaneously cleave the sRNAs and their respective mRNA …