Artificial nuclease-dependent DNA cleavage systems (zinc-finger nuclease, ZFN;
transcription activator like effectors, TALENs) and exogenous nucleic acid defense systems …

The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …

CRISPR loss of function screens are powerful tools to interrogate biology but exhibit a
number of biases and artifacts that can confound the results. Here, we introduce Chronos …

CRISPR-Cas9 viability screens are increasingly performed at a genome-wide scale across
large panels of cell lines to identify new therapeutic targets for precision cancer therapy …

Forward genetic screens are powerful tools for the unbiased discovery and functional
characterization of specific genetic elements associated with a phenotype of interest …

We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA. Here,
we build on this observation with a comprehensive algorithm that incorporates chromatin …

Long noncoding RNAs (lncRNAs) are involved in many physiological and pathological
processes, such as development, aging, immunity, and cancer. Mechanistically, lncRNAs …

CRISPR/Cas9 gene editing can inactivate genes in a precise manner. This process involves
DNA double-strand breaks (DSB), which may incur a loss of cell fitness. We hypothesize that …

The SLC25 carrier family consists of 53 transporters that shuttle nutrients and co-factors
across mitochondrial membranes. The family is highly redundant and their transport …

Abstract Bromodomain-containing protein 9 (BRD9) is a recently identified subunit of
SWI/SNF (BAF) chromatin remodeling complexes, yet its function is poorly understood. Here …