Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications

J Sankaran, T Wohland - Communications Biology, 2023 - nature.com
Fluorescence correlation spectroscopy (FCS) is a single molecule sensitive tool for the
quantitative measurement of biomolecular dynamics and interactions. Improvements in …

Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms

JW Krieger, AP Singh, N Bag, CS Garbe… - Nature protocols, 2015 - nature.com
Single-plane illumination (SPIM) or total internal reflection fluorescence (TIRF) microscopes
can be combined with fast and single-molecule-sensitive cameras to allow spatially resolved …

Imaging fluorescence fluctuation spectroscopy: new tools for quantitative bioimaging

N Bag, T Wohland - Annual review of physical chemistry, 2014 - annualreviews.org
Fluorescence fluctuation spectroscopy (FFS) techniques provide information at the single-
molecule level with excellent time resolution. Usually applied at a single spot in a sample …

Simultaneous spatiotemporal super-resolution and multi-parametric fluorescence microscopy

J Sankaran, H Balasubramanian, WH Tang… - Nature …, 2021 - nature.com
Super-resolution microscopy and single molecule fluorescence spectroscopy require
mutually exclusive experimental strategies optimizing either temporal or spatial resolution …

Fluorescence, phosphorescence, and chemiluminescence

N Siraj, B El-Zahab, S Hamdan, TE Karam… - Analytical …, 2016 - ACS Publications
Fluorescence, Phosphorescence, and Chemiluminescence cover a wide array of topics.
One can simply examine the number of topics that arise from photon induced excitation and …

[图书][B] An introduction to fluorescence correlation spectroscopy

T Wohland, S Maiti, R Macháň - 2020 - iopscience.iop.org
An Introduction to Fluorescence Correlation Spectroscopy represents a comprehensive
introduction to fluorescence correlation spectroscopy (FCS), a biophysical experimental …

[HTML][HTML] Temperature dependence of diffusion in model and live cell membranes characterized by imaging fluorescence correlation spectroscopy

N Bag, DHX Yap, T Wohland - Biochimica et Biophysica Acta (BBA) …, 2014 - Elsevier
The organization of the plasma membrane is regulated by the dynamic equilibrium between
the liquid ordered (L o) and liquid disordered (L d) phases. The abundance of the L o phase …

On the equivalence of FCS and FRAP: simultaneous lipid membrane measurements

R Macháň, YH Foo, T Wohland - Biophysical journal, 2016 - cell.com
Fluorescence correlation spectroscopy (FCS) and fluorescence recovery after
photobleaching (FRAP) are widely used methods to determine diffusion coefficients …

Plasma membrane organization of epidermal growth factor receptor in resting and ligand-bound states

N Bag, S Huang, T Wohland - Biophysical journal, 2015 - cell.com
The spatial arrangement of the epidermal growth factor receptor (EGFR) on the cellular
plasma membrane is one of the prime factors that control its downstream signaling pathways …

Dual-color fluorescence cross-correlation spectroscopy on a single plane illumination microscope (SPIM-FCCS)

JW Krieger, AP Singh, CS Garbe, T Wohland… - Optics express, 2014 - opg.optica.org
Single plane illumination microscopy based fluorescence correlation spectroscopy (SPIM-
FCS) is a new method for imaging FCS in 3D samples, providing diffusion coefficients, flow …