Analysis of transcriptome and epitranscriptome in plants using PacBio Iso-Seq and nanopore-based direct RNA sequencing
L Zhao, H Zhang, MV Kohnen, KVSK Prasad… - Frontiers in …, 2019 - frontiersin.org
Nanopore sequencing from Oxford Nanopore Technologies (ONT) and Pacific BioSciences
(PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) are …
(PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) are …
Methodologies for transcript profiling using long-read technologies
S Oikonomopoulos, A Bayega, S Fahiminiya… - Frontiers in …, 2020 - frontiersin.org
RNA sequencing using next-generation sequencing technologies (NGS) is currently the
standard approach for gene expression profiling, particularly for large-scale high-throughput …
standard approach for gene expression profiling, particularly for large-scale high-throughput …
Direct RNA nanopore sequencing of full-length coronavirus genomes provides novel insights into structural variants and enables modification analysis
Sequence analyses of RNA virus genomes remain challenging owing to the exceptional
genetic plasticity of these viruses. Because of high mutation and recombination rates …
genetic plasticity of these viruses. Because of high mutation and recombination rates …
High-density resolution of the Kaposi's sarcoma associated herpesvirus transcriptome identifies novel transcript isoforms generated by long-range transcription and …
Kaposi's sarcoma-associated herpesvirus is the etiologic agent of Kaposi's sarcoma and two
B-cell malignancies. Recent advancements in sequencing technologies have led to high …
B-cell malignancies. Recent advancements in sequencing technologies have led to high …
Long-read sequencing–a powerful tool in viral transcriptome research
Long-read sequencing (LRS) has become increasingly popular due to its strengths in de
novo assembly and in resolving complex DNA regions as well as in determining full-length …
novo assembly and in resolving complex DNA regions as well as in determining full-length …
Long-read sequencing uncovers a complex transcriptome topology in varicella zoster virus
Background Varicella zoster virus (VZV) is a human pathogenic alphaherpesvirus harboring
a relatively large DNA molecule. The VZV transcriptome has already been analyzed by …
a relatively large DNA molecule. The VZV transcriptome has already been analyzed by …
Challenges in identifying mRNA transcript starts and ends from long-read sequencing data
Long-read sequencing (LRS) technologies have the potential to revolutionize scientific
discoveries in RNA biology through the comprehensive identification and quantification of …
discoveries in RNA biology through the comprehensive identification and quantification of …
Multiple long-read sequencing survey of herpes simplex virus dynamic transcriptome
Long-read sequencing (LRS) has become increasingly important in RNA research due to its
strength in resolving complex transcriptomic architectures. In this regard, currently two LRS …
strength in resolving complex transcriptomic architectures. In this regard, currently two LRS …
Integrative profiling of Epstein–Barr virus transcriptome using a multiplatform approach
Á Fülöp, G Torma, N Moldován, K Szenthe, F Bánáti… - Virology Journal, 2022 - Springer
Abstract Background Epstein–Barr virus (EBV) is an important human pathogenic
gammaherpesvirus with carcinogenic potential. The EBV transcriptome has previously been …
gammaherpesvirus with carcinogenic potential. The EBV transcriptome has previously been …
Direct nanopore sequencing of mRNA reveals landscape of transcript isoforms in apicomplexan parasites
Alternative splicing is a widespread phenomenon in metazoans by which single genes are
able to produce multiple isoforms of the gene product. However, this has been poorly …
able to produce multiple isoforms of the gene product. However, this has been poorly …