Insertion of proteins and lipopolysaccharide into the bacterial outer membrane

I Botos, N Noinaj, SK Buchanan - … Transactions of the …, 2017 - royalsocietypublishing.org
The bacterial outer membrane contains phospholipids in the inner leaflet and
lipopolysaccharide (LPS) in the outer leaflet. Both proteins and LPS must be frequently …

Redefining the bacterial Type I protein secretion system

FJ Hodges, LT Von Vergel, AF Cunningham… - Advances in Microbial …, 2023 - Elsevier
Type I secretion systems (T1SS) are versatile molecular machines for protein transport
across the Gram-negative cell envelope. The archetypal Type I system mediates secretion of …

Dynamic interplay between the periplasmic chaperone SurA and the BAM complex in outer membrane protein folding

B Schiffrin, JM Machin, TK Karamanos… - Communications …, 2022 - nature.com
Correct folding of outer membrane proteins (OMPs) into the outer membrane of Gram-
negative bacteria depends on delivery of unfolded OMPs to the β-barrel assembly …

Hitting with a BAM: selective killing by lectin-like bacteriocins

MGK Ghequire, T Swings, J Michiels, SK Buchanan… - MBio, 2018 - Am Soc Microbiol
Lectin-like bacteriocins (LlpAs) are secreted by proteobacteria and selectively kill strains of
their own or related species, and they are composed of two B-lectin domains with divergent …

Functions of the BamBCDE lipoproteins revealed by bypass mutations in BamA

EM Hart, TJ Silhavy - Journal of bacteriology, 2020 - Am Soc Microbiol
The heteropentomeric β-barrel assembly machine (BAM complex) is responsible for folding
and inserting a diverse array of β-barrel outer membrane proteins (OMPs) into the outer …

The gain-of-function allele bamAE470K bypasses the essential requirement for BamD in β-barrel outer membrane protein assembly

EM Hart, M Gupta, M Wühr… - Proceedings of the …, 2020 - National Acad Sciences
The outer membrane (OM) of gram-negative bacteria confers innate resistance to toxins and
antibiotics. Integral β-barrel outer membrane proteins (OMPs) function to establish and …

Formation of the β-barrel assembly machinery complex in lipid bilayers as seen by solid-state NMR

C Pinto, D Mance, T Sinnige, M Daniëls… - Nature …, 2018 - nature.com
The β-barrel assembly machinery (BAM) is a pentameric complex (BamA–E), which
catalyzes the essential process of β-barrel protein insertion into the outer membrane of E …

Native β-barrel substrates pass through two shared intermediates during folding on the BAM complex

TMA Dos Santos, BD Thomson, MD Marquez… - Proceedings of the …, 2024 - pnas.org
The assembly of β-barrel proteins into membranes is mediated by the evolutionarily
conserved β-barrel assembly machine (BAM) complex. In Escherichia coli, BAM folds …

Hop‐family Helicobacter outer membrane adhesins form a novel class of Type 5‐like secretion proteins with an interrupted β‐barrel domain

F Coppens, G Castaldo, A Debraekeleer… - Molecular …, 2018 - Wiley Online Library
The human stomach pathogen Helicobacter pyloriattaches to healthy and inflamed gastric
tissue through members of a paralogous family of 'Helicobacter outer membrane …

[HTML][HTML] New vectors for urea-inducible recombinant protein production

J Hothersall, A Osgerby, RE Godfrey, TW Overton… - New …, 2022 - Elsevier
We have developed a novel urea-inducible recombinant protein production system by
exploiting the Proteus mirabilis urease ureR-ureD promoter region and the ureR AraC-family …