Recognizing that interaction with the air–water interface (AWI) is a major challenge for cryo-
EM, we first review current approaches designed to avoid it. Of these, immobilizing particles …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Glycosyltransferases of the C superfamily (GT-Cs) are enzymes found in all domains of life.
They catalyse the stepwise synthesis of oligosaccharides or the transfer of assembled …

Approximately two-thirds of the estimated one-billion metric tons of methane produced
annually by methanogens is derived from the cleavage of acetate. Acetate is broken down …

Highlights•Whole-cell and on-lamellae cryo-ET for structural studies of viral
infection.•Inactivation protocols for improved structural preservation and biosafety.•VLP and …

Single-particle cryo-electron microscopy (cryo-EM SPA) has recently emerged as an
exceptionally well-suited technique for determining the structure of membrane proteins …

We applied raw human liver microsome lysate to a holey carbon grid and used cryo-electron
microscopy (cryo-EM) to define its composition. From this sample we identified and …

ABSTRACT Pentraxin-3 (PTX3) is an octameric protein, comprised of eight identical
protomers, that has diverse functions in reproductive biology, innate immunity and cancer …

Biological macromolecules and assemblies precisely rearrange their atomic 3D structures to
execute cellular functions. Understanding the mechanisms by which these molecular …

Methods of transmission electron microscopy (TEM) are typically used to resolve structures
of vitrified biological specimens using both single particle analysis (SPA) and tomographic …