Efficient heterologous expression of cytochrome P450 enzymes in microorganisms for the biosynthesis of natural products

B Hu, X Zhao, E Wang, J Zhou, J Li… - Critical Reviews in …, 2023 - Taylor & Francis
Natural products, a chemically and structurally diverse class of molecules, possess a wide
spectrum of biological activities, have been used therapeutically for millennia, and have …

New frontiers: harnessing pivotal advances in microbial engineering for the biosynthesis of plant-derived terpenoids

MS Belcher, J Mahinthakumar, JD Keasling - Current opinion in …, 2020 - Elsevier
Highlights•Terpenoids are a vast and diverse class of compounds with applications in both
the industrial and human health spaces.•Computational biology and gene discovery enable …

Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii

KJ Lauersen, J Wichmann, T Baier, SC Kampranis… - Metabolic …, 2018 - Elsevier
Photosynthetic microalgae harbor enormous potential as light-driven green-cell factories for
sustainable bio-production of a range of natural and heterologous products such as …

[HTML][HTML] Functional expression and regulation of eukaryotic cytochrome P450 enzymes in surrogate microbial cell factories

P Durairaj, S Li - Engineering Microbiology, 2022 - Elsevier
Cytochrome P450 (CYP) enzymes play crucial roles during the evolution and diversification
of ancestral monocellular eukaryotes into multicellular eukaryotic organisms due to their …

Redox metabolism for improving whole-cell P450-catalysed terpenoid biosynthesis

B Nowrouzi, L Rios-Solis - Critical Reviews in Biotechnology, 2022 - Taylor & Francis
The growing preference for producing cytochrome P450-mediated natural products in
microbial systems stems from the challenging nature of the organic chemistry approaches …

Challenges in the Heterologous Production of Furanocoumarins in Escherichia coli

JL Rodrigues, D Gomes, LR Rodrigues - Molecules, 2022 - mdpi.com
Coumarins and furanocoumarins are plant secondary metabolites with known biological
activities. As they are present in low amounts in plants, their heterologous production …

Systematic engineering pinpoints a versatile strategy for the expression of functional cytochrome P450 enzymes in Escherichia coli cell factories

M Poborsky, C Crocoll, MS Motawie, BA Halkier - Microbial Cell Factories, 2023 - Springer
Production of plant secondary metabolites in engineered microorganisms provides a
scalable and sustainable alternative to their sourcing from nature or through chemical …

[HTML][HTML] Engineering of Yarrowia lipolytica for the production of plant triterpenoids: asiatic, madecassic, and arjunolic acids

JA Arnesen, AB Del Ama, S Jayachandran… - Metabolic Engineering …, 2022 - Elsevier
Several plant triterpenoids have valuable pharmaceutical properties, but their production
and usage is limited since extraction from plants can burden natural resources, and result in …

Engineering Nannochloropsis oceanica for the production of diterpenoid compounds

ZY Du, WW Bhat, E Poliner, S Johnson, C Bertucci… - Mlife, 2023 - Wiley Online Library
Photosynthetic microalgae like Nannochloropsis hold enormous potential as sustainable,
light‐driven biofactories for the production of high‐value natural products such as …

Evolution of interorganismal strigolactone biosynthesis in seed plants

A Zhou, A Kane, S Wu, K Wang, M Santiago, Y Ishiguro… - Science, 2025 - science.org
Strigolactones (SLs) are methylbutenolide molecules derived from β-carotene through an
intermediate carlactonoic acid (CLA). Canonical SLs act as signals to microbes and plants …