Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …

Blood samples are widely used for PCR-based DNA analysis in fields such as diagnosis of
infectious diseases, cancer diagnostics, and forensic genetics. In this study, the mechanisms …

Evolution is essential to the generation of complexity and ultimately life. It relies on the
propagation of the properties, traits, and characteristics that allow an organism to survive in …

Enzymes underpin the processes required for most biotransformations. However, natural
enzymes are often not optimal for biotechnological uses and must be engineered for …

Highlights•Multiple DNA polymerases with divergent properties have been characterized
and engineered.•Varying characteristics of DNA polymerases result in specialized enzymes …

Introduction Glioblastoma (GBM) is the most lethal primary malignant brain tumor in adults
with poor survival due to acquired therapeutic resistance and rapid recurrence. Currently …

The introduction of thermostable polymerases revolutionized the polymerase chain reaction
(PCR) and biotechnology. However, many GC-rich genes cannot be PCR-amplified with …

Increasing the accuracy of pathogen identification and reducing the duration of analysis
remain relevant for modern molecular diagnostics up to this day. In laboratory and clinical …

DNA polymerases are essential enzymes that play a key role in living organisms, as they
participate in the synthesis and maintenance of the DNA molecule. The intrinsic properties of …