A guide to single-particle tracking
Individual proteins and protein complexes undergo various motion types, including free
diffusion, confinement, subdiffusion and directed motion. Different motion behaviours reflect …
diffusion, confinement, subdiffusion and directed motion. Different motion behaviours reflect …
Probing cellular events, one quantum dot at a time
Monitoring the behavior of single molecules in living cells is a powerful approach to
investigate the details of cellular processes. Owing to their optical, chemical and …
investigate the details of cellular processes. Owing to their optical, chemical and …
Mean square displacement analysis of single-particle trajectories with localization error: Brownian motion in an isotropic medium
X Michalet - Physical Review E—Statistical, Nonlinear, and Soft …, 2010 - APS
We examine the capability of mean square displacement (MSD) analysis to extract reliable
values of the diffusion coefficient D of a single particle undergoing Brownian motion in an …
values of the diffusion coefficient D of a single particle undergoing Brownian motion in an …
Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes
Although the highly dynamic and mosaic organization of the plasma membrane is well-
recognized, depicting a resolved, global view of this organization remains challenging. We …
recognized, depicting a resolved, global view of this organization remains challenging. We …
mGluR5 is transiently confined in perisynaptic nanodomains to shape synaptic function
N Scheefhals, M Westra, HD MacGillavry - Nature communications, 2023 - nature.com
The unique perisynaptic distribution of postsynaptic metabotropic glutamate receptors
(mGluRs) at excitatory synapses is predicted to directly shape synaptic function, but …
(mGluRs) at excitatory synapses is predicted to directly shape synaptic function, but …
GPI-anchored receptor clusters transiently recruit Lyn and Gα for temporary cluster immobilization and Lyn activation: single-molecule tracking study 1
The signaling mechanisms for glycosylphosphatidylinositol-anchored receptors (GPI-ARs)
have been investigated by tracking single molecules in living cells. Upon the engagement or …
have been investigated by tracking single molecules in living cells. Upon the engagement or …
Imaging, tracking and computational analyses of virus entry and egress with the cytoskeleton
Viruses have a dual nature: particles are “passive substances” lacking chemical energy
transformation, whereas infected cells are “active substances” turning-over energy. How …
transformation, whereas infected cells are “active substances” turning-over energy. How …
A hidden Markov model for single particle tracks quantifies dynamic interactions between LFA-1 and the actin cytoskeleton
The extraction of hidden information from complex trajectories is a continuing problem in
single-particle and single-molecule experiments. Particle trajectories are the result of …
single-particle and single-molecule experiments. Particle trajectories are the result of …
Quantitative evaluation of methods to analyze motion changes in single-particle experiments
G Muñoz-Gil, H Bachimanchi, J Pineda… - arXiv preprint arXiv …, 2023 - arxiv.org
The analysis of live-cell single-molecule imaging experiments can reveal valuable
information about the heterogeneity of transport processes and interactions between cell …
information about the heterogeneity of transport processes and interactions between cell …
Automatic detection of diffusion modes within biological membranes using back-propagation neural network
P Dosset, P Rassam, L Fernandez, C Espenel… - BMC …, 2016 - Springer
Background Single particle tracking (SPT) is nowadays one of the most popular technique to
probe spatio-temporal dynamics of proteins diffusing within the plasma membrane. Indeed …
probe spatio-temporal dynamics of proteins diffusing within the plasma membrane. Indeed …