Critical review of 16S rRNA gene sequencing workflow in microbiome studies: From primer selection to advanced data analysis
A Regueira‐Iglesias, C Balsa‐Castro… - Molecular Oral …, 2023 - Wiley Online Library
The multi‐batch reanalysis approach of jointly reevaluating gene/genome sequences from
different works has gained particular relevance in the literature in recent years. The large …
different works has gained particular relevance in the literature in recent years. The large …
The salivary microbiome as a diagnostic biomarker of periodontitis: a 16S multi-batch study before and after the removal of batch effects
A Regueira-Iglesias, B Suárez-Rodríguez… - Frontiers in Cellular …, 2024 - frontiersin.org
Introduction Microbiome-based clinical applications that improve diagnosis related to oral
health are of great interest to precision dentistry. Predictive studies on the salivary …
health are of great interest to precision dentistry. Predictive studies on the salivary …
The Archaea domain: exploring historical and contemporary perspectives with in silico primer coverage analysis for future research in Dentistry
JA de Cena, A Belmok, CM Kyaw… - Archives of Oral Biology, 2024 - Elsevier
Objective The complete picture of how the human microbiome interacts with its host is still
largely unknown, particularly concerning microorganisms beyond bacteria. Although …
largely unknown, particularly concerning microorganisms beyond bacteria. Although …
[HTML][HTML] Comparing the Microbiome of the Adenoids in Children with Secretory Otitis Media and Children without Middle Ear Effusion
O Sokolovs-Karijs, M Brīvība, R Saksis, M Rozenberga… - Microorganisms, 2024 - mdpi.com
Background: The adenoids, primary sites of microbial colonization in the upper airways, can
influence the development of various conditions, including otitis media with effusion (OME) …
influence the development of various conditions, including otitis media with effusion (OME) …
Poultry Abattoir Site Microbiome Analysis Reveals a Core, Time-Independent Processor Microbiota
Meat processing facilities play a vital role in the establishment of the end-product microbiota,
from control steps implemented to control pathogens to adulterant exposure to the …
from control steps implemented to control pathogens to adulterant exposure to the …
Impact of 16S rRNA gene redundancy and primer pair selection on the quantification and classification of oral microbiota in next-generation sequencing
A Regueira-Iglesias, L Vázquez-González… - Microbiology …, 2023 - Am Soc Microbiol
This study aimed to evaluate the number of 16S rRNA genes in the complete genomes of
the bacterial and archaeal species inhabiting the human mouth and to assess how the use …
the bacterial and archaeal species inhabiting the human mouth and to assess how the use …
Developing the script “degenerate primer 111” to enhance the coverage of universal primers for the small subunit rRNA gene on target microorganisms
Z Qin, X Xu, F Xu, Y Zhang, P Su, C Shen - Frontiers in Microbiology, 2024 - frontiersin.org
Amplifying small subunit (SSU) rRNA genes with universal primers in assessing microbial
populations diversity, but target microorganisms are sometimes omitted due to inadequate …
populations diversity, but target microorganisms are sometimes omitted due to inadequate …
Ribotyping Staphylococcus epidermidis Using Probabilistic Sequence Analysis and Levenshtein Distance Algorithm
RY Huang, C Zhang, HL Lim - Current Microbiology, 2025 - Springer
Staphylococcus epidermidis (S. epidermidis) live in different human locations and natural
environments. For ribotyping S. epidermidis sub-species, 2507 PCR-amplified reads of 16S …
environments. For ribotyping S. epidermidis sub-species, 2507 PCR-amplified reads of 16S …
Optimal 16S rRNA gene amplicon sequencing analysis for oral microbiota to avoid the potential bias introduced by trimming length, primer, and database
T Nagai, T Shiba, K Komatsu, T Watanabe… - Microbiology …, 2024 - journals.asm.org
ABSTRACT 16S rRNA gene amplicon sequencing analysis is used to investigate bacterial
communities; however, the estimated bacterial composition can differ from the original due …
communities; however, the estimated bacterial composition can differ from the original due …
[PDF][PDF] 食品防腐剂对口腔菌群和口腔健康影响的研究进展
谢好卉， 周碧颖， 张雨桐， 马丹丹 - 中国微生态学杂志, 2024 - cjm.dmu.edu.cn
食品防腐剂是一种广泛应用于食品加工和储存中的化学物质, 其主要作用是抑制微生物的生长和
繁殖, 从而延长食品的保质期. 口腔菌群是仅次于肠道菌群的人体第二大微生物群 …
繁殖, 从而延长食品的保质期. 口腔菌群是仅次于肠道菌群的人体第二大微生物群 …