Cracking the code: reprogramming the genetic script in prokaryotes and eukaryotes to harness the power of noncanonical amino acids

C Jann, S Giofré, R Bhattacharjee, EA Lemke - Chemical Reviews, 2024 - ACS Publications
Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …

Advances of azide-alkyne cycloaddition-click chemistry over the recent decade

MS Singh, S Chowdhury, S Koley - Tetrahedron, 2016 - Elsevier
During the past years, a variety of scientific and methodological developments have been
achieved, which urge chemists to increase the tools of their arsenal to improve the ease and …

Metal-catalyzed azide-alkyne “click” reactions: Mechanistic overview and recent trends

C Wang, D Ikhlef, S Kahlal, JY Saillard… - Coordination Chemistry …, 2016 - Elsevier
This review is focused on the mechanistic aspects and recent trends of the metal-catalyzed
azide-alkyne cycloaddition (MAAC) so-called “click” reactions with catalysts based on …

Advances in chemical probing of protein O-GlcNAc glycosylation: structural role and molecular mechanisms

A Saha, D Bello, A Fernández-Tejada - Chemical Society Reviews, 2021 - pubs.rsc.org
The addition of O-linked-β-DN-acetylglucosamine (O-GlcNAc) onto serine and threonine
residues of nuclear and cytoplasmic proteins is an abundant, unique post-translational …

Mechanisms and substituent effects of metal-free bioorthogonal reactions

T Deb, J Tu, RM Franzini - Chemical reviews, 2021 - ACS Publications
Reactions that occur under physiological conditions find diverse uses in the chemical and
biological sciences. However, the limitations that biological systems place on chemical …

Photoclick chemistry: a bright idea

BD Fairbanks, LJ Macdougall, S Mavila, J Sinha… - Chemical …, 2021 - ACS Publications
At its basic conceptualization, photoclick chemistry embodies a collection of click reactions
that are performed via the application of light. The emergence of this concept has had …

Fluorescent amino acids as versatile building blocks for chemical biology

Z Cheng, E Kuru, A Sachdeva, M Vendrell - Nature Reviews Chemistry, 2020 - nature.com
Fluorophores have transformed the way we study biological systems, enabling non-invasive
studies in cells and intact organisms, which increase our understanding of complex …

Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs)

DP Murale, SC Hong, MM Haque, JS Lee - Proteome Science, 2016 - Springer
Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that
are crucial for biomedical research and drug discovery. Various techniques have been used …

An overview of chemo-and site-selectivity aspects in the chemical conjugation of proteins

C Sornay, V Vaur, A Wagner… - Royal Society Open …, 2022 - royalsocietypublishing.org
The bioconjugation of proteins—that is, the creation of a covalent link between a protein and
any other molecule—has been studied for decades, partly because of the numerous …

Biocatalysis with unnatural amino acids: enzymology meets xenobiology

F Agostini, JS Völler, B Koksch… - Angewandte Chemie …, 2017 - Wiley Online Library
The goal of xenobiology is to design biological systems endowed with unusual biochemical
functions, whereas enzymology concerns the study of enzymes, the workhorses of …