CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) have become
ubiquitous approaches to control gene expression in bacteria due to their simple design and …

Tuberculosis (TB) is a devastating infectious disease that kills over a million people every
year. There is an increasing burden of multi drug resistance (MDR) and extensively drug …

Antibiotic resistance in Mycobacterium tuberculosis exclusively originates from
chromosomal mutations, either during normal DNA replication or under stress, when the …

DNA repair systems allow microbes to survive in diverse environments that compromise
chromosomal integrity. Pathogens such as Mycobacterium tuberculosis must contend with …

Nucleotide excision repair (NER) is one of the most extensively studied DNA repair
processes in both prokaryotes and eukaryotes. The NER pathway is a highly conserved …

It was recently reported that 4-substituted picolinohydrazonamides carrying hydrophilic
cyclic amines, such as morpholine and pyrrolidine, at the end of their thiosemicarbazide …

Mycobacterium smegmatis Nei2 is a monomeric enzyme with AP β-lyase activity on single-
stranded DNA. Expression of Nei2, and its operonic neighbor Lhr (a tetrameric 3′-to-5 …

Mycobacterium smegmatis Lhr exemplifies a novel clade of helicases composed of an N-
terminal ATPase/helicase domain (Lhr-Core) and a large C-terminal domain (Lhr-CTD) that …

Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB), is a serious contagious
disease. MTB-encoded small regulatory RNA (sRNA) MTS2823 was reported to be …

Abstract In Escherichia coli, RecA plays a central role in the rescue of stalled replication
forks, double-strand break (DSB) repair, homologous recombination (HR), and induction of …