l Red-mediated genetic manipulation of antibiotic-producing Streptomyces

B Gust, G Chandra, D Jakimowicz… - Advances in applied …, 2004 - books.google.com
Streptomycetes are high G+ C Gram-positive, antibiotic-producing, soil bacteria that undergo
complex morphological differentiation (Chater, 2001). The 8.7 Mb Streptomyces coelicolor …

[PDF][PDF] Insights on Escherichia coli biofilm formation and inhibition from whole‐transcriptome profiling

TK Wood - Environmental microbiology, 2009 - Wiley Online Library
Biofilms transform independent cells into specialized cell communities. Here are presented
some insights into biofilm formation ascertained with the best‐characterized strain …

Conversion of Escherichia coli to generate all biomass carbon from CO2

S Gleizer, R Ben-Nissan, YM Bar-On, N Antonovsky… - Cell, 2019 - cell.com
The living world is largely divided into autotrophs that convert CO 2 into biomass and
heterotrophs that consume organic compounds. In spite of widespread interest in renewable …

Bacterial retrons encode phage-defending tripartite toxin–antitoxin systems

J Bobonis, K Mitosch, A Mateus, N Karcher, G Kritikos… - Nature, 2022 - nature.com
Retrons are prokaryotic genetic retroelements encoding a reverse transcriptase that
produces multi-copy single-stranded DNA (msDNA). Despite decades of research on the …

Converting Escherichia coli to a synthetic methylotroph growing solely on methanol

FYH Chen, HW Jung, CY Tsuei, JC Liao - Cell, 2020 - cell.com
Methanol, being electron rich and derivable from methane or CO 2, is a potentially
renewable one-carbon (C1) feedstock for microorganisms. Although the ribulose …

Mucispirillum schaedleri antagonizes Salmonella virulence to protect mice against colitis

S Herp, S Brugiroux, D Garzetti, D Ring, LM Jochum… - Cell host & …, 2019 - cell.com
The microbiota and the gastrointestinal mucus layer play a pivotal role in protection against
non-typhoidal Salmonella enterica serovar Typhimurium (S. Tm) colitis. Here, we analyzed …

RNA-RNA interactomes of ProQ and Hfq reveal overlapping and competing roles

S Melamed, PP Adams, A Zhang, H Zhang, G Storz - Molecular cell, 2020 - cell.com
Base pairing RNAs modulate gene expression in all studied organisms. In many bacteria,
the base pairing between most small regulatory RNAs (sRNAs) and their targets is mediated …

Genomic aberrations after short-term exposure to colibactin-producing E. coli transform primary colon epithelial cells

A Iftekhar, H Berger, N Bouznad, J Heuberger… - Nature …, 2021 - nature.com
Genotoxic colibactin-producing pks+ Escherichia coli induce DNA double-strand breaks,
mutations, and promote tumor development in mouse models of colorectal cancer (CRC) …

Identifying and visualizing functional PAM diversity across CRISPR-Cas systems

RT Leenay, KR Maksimchuk, RA Slotkowski… - Molecular cell, 2016 - cell.com
CRISPR-Cas adaptive immune systems in prokaryotes boast a diversity of protein families
and mechanisms of action, where most systems rely on protospacer-adjacent motifs (PAMs) …

Phase separation in the outer membrane of Escherichia coli

G Benn, IV Mikheyeva, PG Inns… - Proceedings of the …, 2021 - National Acad Sciences
Gram-negative bacteria are surrounded by a protective outer membrane (OM) with
phospholipids in its inner leaflet and lipopolysaccharides (LPS) in its outer leaflet. The OM is …