The advent of high throughput genome‐scale bioinformatics has led to an exponential
increase in available cellular system data. Systems metabolic engineering attempts to use …

Metabolic flux analysis is a vital tool used to determine the ultimate output of cellular
metabolism and thus detect biotechnologically relevant bottlenecks in productivity. 13C …

Interleukin-2 has emerged as a potent protein-based drug to treat various cancers, AIDS,
and autoimmune diseases. Despite its immense requirement, the production procedures are …

The cell culture medium is an intricate mixture of components which has a tremendous effect
on cell growth and recombinant protein production. Regular cell culture medium includes …

Background: Human alpha 1-antitrypsin (AAT) is a monomeric glycosylated protein; it is the
potent inhibitor of a whole range of serine proteases and protects tissues against their …

Developing novel methanol-feeding strategies for the improved production of heterologous
proteins in high cell-density fed-batch cultures of Pichia pastoris has been of great interest …

The high cell density culture of baker's yeast FX‐2 was investigated in a 50 L (A) automatic
bioreactor. Herein, it was found firstly that the Crabtree effect clearly existed in batch …

The expression of rhIL‐2 (recombinant human interleukin‐2) in bacteria results in the
formation of insoluble inclusion‐body aggregates. These aggregates were first solubilized …

Spent fermentation media from bioprocessing represent a significant waste stream, and
interest in recycling them as part of the developing circular bioeconomy is growing. The …

A novel amino acid supplementation strategy was developed for enhancing the production
of IL‐2 (interleukin‐2; as a model protein) by recombinant Escherichia coli BL21 (pET21a …