Nuclei are intricately structured, and nuclear metabolism has an elaborate spatial
organization. The architecture of the nucleus includes two overlapping and nucleic-acid …

Publisher Summary This chapter focuses on the evidence for loops in the genome and on
the potential function of this higher-order folding. The loop hypothesis for the organization of …

We have obtained a polyclonal antibody that recognizes a major polypeptide component of
chicken mitotic chromosome scaffolds. This polypeptide migrates in SDS PAGE with Mr …

The nucleolus had consistently attracted the attention of investigators in the fields of cell
biology and pathology. Because of its ubiquitous presence in the nucleus of eukaryotic cells …

One level of DNA organization in metaphase chromosomes is brought about by a
scaffolding structure that is stabilized by metalloprotein interactions. Fast-sedimenting …

SC1 is a prominent, 170,000 M r, non-histone protein of HeLa metaphase chromosomes.
This protein binds DNA and was previously identified as one of the major structural …

We have developed a simple in vitro system for studying phosphorylation in isolated HeLa
metaphase chromosomes which utilizes the endogenous protein kinase and …

Nucleic acid metabolism is structurally organized in the nucleus. DNA replication and
transcription have been localized to particular nuclear domains. Additional domains have …

Two forms of histone-depleted HeLa nuclei have been characterized (Lebkowski & Laemmli,
1982). In the studies presented here, the proteins associated with both structures are …

The long-range order of the DNA in interphase nuclei was investigated by sedimentation
studies. From these studies, two discreet sedimentation forms have been characterized for …