Hardwood species are valuable biological resources that have an important role in the
economy and ecology of ecosystems worldwide. Non-zygotic or somatic embryogenesis …

Quercus ilex (holm oak) is one of the most representative trees in the Mediterranean basin,
but now the sustainability of its ecosystems is at serious risk due to the lack of natural …

This paper reports the successful micropropagation of mature Quercus ilex trees known as
reluctant to in vitro propagation. Crown branch segments collected from 30 to 100 year-old …

The CRISPR/Cas9 ribonucleoprotein (RNP)-mediated technology represents a fascinating
tool for modifying gene expression or mutagenesis as this system allows for obtaining …

Somatic embryogenesis is considered a convenient tool for investigating the regulating
mechanisms of embryo formation; it is also a feasible system for in vitro regeneration …

Somatic embryogenesis from in vitro leaf and shoot apex explants excised from axillary
shoot cultures established from two mature Quercus ilex trees has been developed. Somatic …

Holm oak populations have deteriorated drastically due to oak decline syndrome. The first
objective of the present study was to investigate the use of axillary budding and somatic …

In somatic embryogenic systems of various oak species, including Quercus alba and Q.
rubra, embryogenic lines generally proliferate through generation of nodular embryogenic …

Key message Integuments from holm oak developing ovules were suitable initial explants to
obtain embryogenic lines from which plants could be regenerated. Abstract The …

Holm oak (Quercus ilex) is one of the most widely distributed tree species in the
Mediterranean basin. High mortality rates have been observed in holm oak populations in …