Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC–
HRMS) variants currently represent the best tools to tackle the challenges of complexity and …

Mass spectrometry (MS)-based proteomics aims to characterize comprehensive proteomes
in a fast and reproducible manner. Here we present the narrow-window data-independent …

This protocol describes the use of TurboID and split-TurboID in proximity labeling
applications for mapping protein–protein interactions and subcellular proteomes in live …

MS-based proteome profiling has become increasingly comprehensive and quantitative, yet
a persistent shortcoming has been the relatively large samples required to achieve an in …

The growing trend toward high-throughput proteomics demands rapid liquid
chromatography–mass spectrometry (LC–MS) cycles that limit the available time to gather …

Mass spectrometry is unmatched in its versatility for studying practically any aspect of the
proteome. Because the foundations of mass spectrometry-based proteomics are complex …

In recent decades, mass spectrometry has moved more than ever before into the front line of
protein-centered research. After being established at the qualitative level, the more …

In bottom-up proteomics, peptides are separated by liquid chromatography with elution peak
widths in the range of seconds, whereas mass spectra are acquired in about 100 …

Proteomics researchers today face an interesting challenge: how to choose among the
dozens of data processing and analysis pipelines available for converting tandem mass …