Genetically encoded fluorescent biosensors illuminate the spatiotemporal regulation of signaling networks

EC Greenwald, S Mehta, J Zhang - Chemical reviews, 2018 - ACS Publications
Cellular signaling networks are the foundation which determines the fate and function of
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …

Fluorescent proteins as genetically encoded FRET biosensors in life sciences

B Hochreiter, A Pardo Garcia, JA Schmid - Sensors, 2015 - mdpi.com
Fluorescence-or Förster resonance energy transfer (FRET) is a measurable physical energy
transfer phenomenon between appropriate chromophores, when they are in sufficient …

Noninvasive imaging of tumor glycolysis and chemotherapeutic resistance via de novo design of molecular afterglow scaffold

L Lei, F Yang, X Meng, L Xu, P Liang… - Journal of the …, 2023 - ACS Publications
Chemotherapeutic resistance poses a significant challenge in cancer treatment, resulting in
the reduced efficacy of standard chemotherapeutic agents. Abnormal metabolism …

Improving FRET dynamic range with bright green and red fluorescent proteins

AJ Lam, F St-Pierre, Y Gong, JD Marshall, PJ Cranfill… - Nature …, 2012 - nature.com
A variety of genetically encoded reporters use changes in fluorescence (or Förster)
resonance energy transfer (FRET) to report on biochemical processes in living cells. The …

Development of an optimized backbone of FRET biosensors for kinases and GTPases

N Komatsu, K Aoki, M Yamada… - Molecular biology of …, 2011 - Am Soc Cell Biol
Biosensors based on the principle of Förster (or fluorescence) resonance energy transfer
(FRET) have shed new light on the spatiotemporal dynamics of signaling molecules. Among …

[图书][B] FRET-Förster resonance energy transfer: from theory to applications

IL Medintz, N Hildebrandt - 2013 - books.google.com
FRET–Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed
primer on all aspects of the topic, this ready reference reflects the incredible expansion in …

Single-cell resolved imaging reveals intra-tumor heterogeneity in glycolysis, transitions between metabolic states, and their regulatory mechanisms

H Kondo, CDH Ratcliffe, S Hooper, J Ellis, JI MacRae… - Cell reports, 2021 - cell.com
Inter-cellular heterogeneity in metabolic state has been proposed to influence many cancer
phenotypes, including responses to targeted therapy. Here, we track the transitions and …

Use of luciferase probes to measure ATP in living cells and animals

G Morciano, AC Sarti, S Marchi, S Missiroli, S Falzoni… - Nature protocols, 2017 - nature.com
ATP, the energy exchange factor that connects anabolism and catabolism, is required for
major reactions and processes that occur in living cells, such as muscle contraction …

Single cell optical imaging and spectroscopy

AS Stender, K Marchuk, C Liu, S Sander… - Chemical …, 2013 - ACS Publications
In his 1665 treatise, Micrographia, Robert Hooke described the many observations he had
made using a microscope, including compartment-like structures in cork samples that he …

A versatile toolkit to produce sensitive FRET biosensors to visualize signaling in time and space

RD Fritz, M Letzelter, A Reimann, K Martin, L Fusco… - Science …, 2013 - science.org
Genetically encoded, ratiometric biosensors based on fluorescence resonance energy
transfer (FRET) are powerful tools to study the spatiotemporal dynamics of cell signaling …