Immortalized bovine satellite cells for cultured meat applications

AJ Stout, MJ Arnett, K Chai, T Guo, L Liao… - ACS synthetic …, 2023 - ACS Publications
For cultured meat to succeed at scale, muscle cells from food-relevant species must be
expanded in vitro in a rapid and reliable manner to produce millions of metric tons of …

HPV E6 inhibits E6AP to regulate epithelial homeostasis by modulating keratinocyte differentiation commitment and YAP1 activation

W Yin, N Egawa, K Zheng, H Griffin, P Tian… - PLoS …, 2023 - journals.plos.org
Human papillomaviruses (HPV) cause persistent infections by modulating epithelial
homeostasis in cells of the infected basal layer. Using FUCCI and cell-cell competition …

Immortalized canine adipose-derived mesenchymal stem cells as a novel candidate cell source for mesenchymal stem cell therapy

Y Yasumura, T Teshima, T Nagashima… - International Journal of …, 2023 - mdpi.com
Mesenchymal stem cells are expected to be a cell source for stem cell therapy of various
diseases in veterinary medicine. However, donor-dependent cell heterogenicity has been a …

Efficient immortalization of human dental pulp stem cells with expression of cell cycle regulators with the intact chromosomal condition

A Orimoto, S Kyakumoto, T Eitsuka, K Nakagawa… - PLoS …, 2020 - journals.plos.org
Clinical studies have recently demonstrated that autologous transplantation of mobilized
dental pulp stem cells is a safe and efficacious potential therapy for pulp regeneration …

Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas

T Nukpook, T Ekalaksananan, T Kiyono, P Kasemsiri… - Scientific Reports, 2021 - nature.com
To better understand the pathogenesis of nasal polyps (NPs) and sinonasal inverted
papillomas (SIPs), we aimed to establish cell lines from fresh tissues of NPs and SIPs and …

The effects of immortalization on the N-glycome and proteome of CDK4-transformed lung cancer cells

MRS Alvarez, PG Moreno, SJB Grijaldo-Alvarez… - …, 2024 - academic.oup.com
Biological experiments are often conducted in vitro using immortalized cells due to their
accessibility and ease of propagation compared to primary cells and live animals. However …

Combinatorial expression of cell cycle regulators is more suitable for immortalization than oncogenic methods in dermal papilla cells

T Fukuda, K Furuya, K Takahashi, A Orimoto, E Sugano… - IScience, 2021 - cell.com
The immortalized cell is an essential research tool that uses robust growth properties for the
functional investigation of gene products. Immortalized mammalian cells have mainly been …

Primary and immortalized cell lines derived from the Amami rabbit (Pentalagus furnessi) and evolutionally conserved cell cycle control with CDK4 and Cyclin D1

A Orimoto, M Katayama, T Tani, K Ito, T Eitsuka… - Biochemical and …, 2020 - Elsevier
Abstract The Amami rabbit (Pentagulus furnessi) is a dark brown-furred rabbit classified as
an endangered species and only found in the Amami Islands of Japan. They are often called …

Human derived immortalized dermal papilla cells with a constant expression of testosterone receptor

T Fukuda, K Takahashi, S Takase, A Orimoto… - Frontiers in Cell and …, 2020 - frontiersin.org
Androgenetic alopecia (AGA) is the most common type of hair loss, and is mainly caused by
the biological effects of testosterone on dermal papilla cells (DPCs). In vitro culturing of …

Sheep‐derived cell immortalization through the expression of cell cycle regulators and biological characterization using transcriptomes

N Kikuchi, H Matsusaka, L Bai, H Sano… - Cell Biology …, 2023 - Wiley Online Library
Sheep are important domestic animals for the production of wool and meat. Although
numerous cultured cell lines from humans and mice have been established, the number of …