Biological membranes and membrane proteins, responsible for numerous exciting
biological processes, present one of the paramount challenges in biophysics today …

Unlike water soluble proteins, the structures of helicaltransmembrane proteins depend on a
very complex environment. These proteins sit in the midst of dramatic electrical and …

Magic angle spinning solid-state NMR (MAS ssNMR) spectroscopy is a powerful method for
atomic-resolution structure determination of biomacromolecules that are recalcitrant to …

Phospholamban (PLN) is a mini-membrane protein that directly controls the cardiac Ca2+-
transport response to β-adrenergic stimulation, thus modulating cardiac output during the …

The sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA) plays a central role in muscle
contractility and nonshivering thermogenesis. SERCA is regulated by sarcolipin (SLN), a …

Oriented-sample solid-state NMR represents one of few experimental methods capable of
characterising the membrane-bound conformation of proteins in the cell membrane. Since …

SERCA is a P-type ATPase embedded in the sarcoplasmic reticulum and plays a central
role in muscle relaxation. SERCA's function is regulated by single-pass membrane proteins …

Using the DUMAS (Dual acquisition Magic Angle Spinning) solid-state NMR approach, we
created new pulse schemes that enable the simultaneous acquisition of three dimensional …

We propose a general method that enables the acquisition of multiple 2D and 3D solid-state
NMR spectra for U-13 C, 15 N-labeled proteins. This method, called MEIOSIS (Multiple …

Oriented solid-state NMR is the most direct methodology to obtain the orientation of
membrane proteins with respect to the lipid bilayer. The method consists of measuring 1 H …