Metabolite measurement: pitfalls to avoid and practices to follow
Metabolites are the small biological molecules involved in energy conversion and
biosynthesis. Studying metabolism is inherently challenging due to metabolites' reactivity …
biosynthesis. Studying metabolism is inherently challenging due to metabolites' reactivity …
Mass spectrometric based approaches in urine metabolomics and biomarker discovery
Urine metabolomics has recently emerged as a prominent field for the discovery of non‐
invasive biomarkers that can detect subtle metabolic discrepancies in response to a specific …
invasive biomarkers that can detect subtle metabolic discrepancies in response to a specific …
Human pancreatic cancer tumors are nutrient poor and tumor cells actively scavenge extracellular protein
Glucose and amino acids are key nutrients supporting cell growth. Amino acids are imported
as monomers, but an alternative route induced by oncogenic KRAS involves uptake of …
as monomers, but an alternative route induced by oncogenic KRAS involves uptake of …
Metabolomics of Mycobacterium tuberculosis reveals compartmentalized co-catabolism of carbon substrates
LPS de Carvalho, SM Fischer, J Marrero, C Nathan… - Chemistry & biology, 2010 - cell.com
Metabolic adaptation to the host environment is a defining feature of the pathogenicity of
Mycobacterium tuberculosis (Mtb), but we lack biochemical knowledge of its metabolic …
Mycobacterium tuberculosis (Mtb), but we lack biochemical knowledge of its metabolic …
Multifunctional essentiality of succinate metabolism in adaptation to hypoxia in Mycobacterium tuberculosis
H Eoh, KY Rhee - Proceedings of the National Academy of …, 2013 - National Acad Sciences
Mycobacterium tuberculosis is a chronic, facultative intracellular pathogen that spends the
majority of its decades-long life cycle in a non-or slowly replicating state. However, the …
majority of its decades-long life cycle in a non-or slowly replicating state. However, the …
Gluconeogenic carbon flow of tricarboxylic acid cycle intermediates is critical for Mycobacterium tuberculosis to establish and maintain infection
J Marrero, KY Rhee, D Schnappinger… - Proceedings of the …, 2010 - National Acad Sciences
Metabolic adaptation to the host niche is a defining feature of the pathogenicity of
Mycobacterium tuberculosis (Mtb). In vitro, Mtb is able to grow on a variety of carbon …
Mycobacterium tuberculosis (Mtb). In vitro, Mtb is able to grow on a variety of carbon …
Avoiding misannotation of in-source fragmentation products as cellular metabolites in liquid chromatography–mass spectrometry-based metabolomics
Liquid chromatography–mass spectrometry (LC-MS) technology allows for rapid quantitation
of cellular metabolites, with metabolites identified by mass spectrometry and …
of cellular metabolites, with metabolites identified by mass spectrometry and …
Expanding coverage of the metabolome for global metabolite profiling
Mass spectrometry-based metabolomics is the comprehensive study of naturally occurring
small molecules collectively known as the metabolome. Given the vast structural diversity …
small molecules collectively known as the metabolome. Given the vast structural diversity …
Para-Aminosalicylic Acid Acts as an Alternative Substrate of Folate Metabolism in Mycobacterium tuberculosis
Folate biosynthesis is an established anti-infective target, and the antifolate para-
aminosalicylic acid (PAS) was one of the first anti-infectives introduced into clinical practice …
aminosalicylic acid (PAS) was one of the first anti-infectives introduced into clinical practice …
Evaluation of coupling reversed phase, aqueous normal phase, and hydrophilic interaction liquid chromatography with Orbitrap mass spectrometry for metabolomic …
In this study, we assessed three liquid chromatographic platforms: reversed phase (RP),
aqueous normal phase (ANP), and hydrophilic interaction (HILIC) for the analysis of polar …
aqueous normal phase (ANP), and hydrophilic interaction (HILIC) for the analysis of polar …