Cryo-electron microscopy (cryo-EM) of single-particle specimens is used to determine the
structure of proteins and macromolecular complexes without the need for crystals. Recent …

3D cryo-electron microscopy (cryo-EM) is an expanding structural biology technique that has
recently undergone a quantum leap progression in its achievable resolution and its …

Single-particle cryo-electron microscopy (cryo-EM) has emerged over the last two decades
as a technique capable of studying challenging systems that otherwise defy structural …

Abstract Three-dimensional (3D) structure determination by single particle electron
cryomicroscopy (cryoEM) involves the calculation of an initial 3D model, followed by …

Helical reconstruction in RELION is increasingly being used to determine the atomic
structures of amyloid filaments from electron cryo-microscopy (cryo-EM) images. However …

A computational procedure is described for assigning the absolute hand of the structure of a
protein or assembly determined by single-particle electron microscopy. The procedure …

Oxidative phosphorylation (OXPHOS) is the main source of energy in eukaryotic cells. This
process is performed by means of electron flow between four enzymes, of which three are …

In recent years, an abundance of new molecular structures have been elucidated using cryo-
electron microscopy (cryo-EM), largely due to advances in hardware technology and data …

Cryoelectron microscopy of biological molecules is among the hottest growth areas in
biophysics and structural biology at present, and Frank is arguably the most distinguished …

One of the aims of modern microscopy is to quantify two-, three-, or even four-dimensional
phenomena in biology, medicine, and material sciences. The requirements imposed on …