Fluorescent proteins have revolutionized biomedical research as they are easy to use for
protein tagging, cope without fixation or permeabilization, and thus, enable live cell imaging …

The widespread use of Cas12a (formerly Cpf1) nucleases for genome engineering is limited
by their requirement for a rather long TTTV protospacer adjacent motif (PAM) sequence …

Background The propensity for off-target activity of Streptococcus pyogenes Cas9 (SpCas9)
has been considerably decreased by rationally engineered variants with increased fidelity …

Despite the recent discover of genome-editing methods, today we can say these
approaches have firmly entered our life. Two approaches—knocking out malfunctioning …

Background Efficient and convenient genome-editing toolkits can expedite genomic
research and strain improvement for desirable phenotypes. Zymomonas mobilis is a highly …

Increased fidelity mutants of the SpCas9 nuclease constitute the most promising approach to
mitigating its off-target effects. However, these variants are effective only in a restricted target …

CRISPR/Cas9-based genome editing has dramatically accelerated genome engineering.
An important aspect of genome engineering is efficient knock-in technology. For improved …

Adenine and cytosine base editors (ABE, CBE) allow for precision genome engineering.
Here, Base Editor Activity Reporter (BEAR), a plasmid-based fluorescent tool is introduced …

Functional annotation of the vast noncoding landscape of the diploid human genome still
remains a major challenge of genomic research. An efficient, scarless, biallelic, and gene …

Expression of the ABCG2 multidrug transporter is a marker of cancer stem cells and a
predictor of recurrent malignant disease. Understanding how human ABCG2 expression is …