Native mass spectrometry of membrane proteins
Biological membranes formed of amphipathic lipid bilayers provide chemical barriers to
separate cells from their external environments and define subcellular compartments …
separate cells from their external environments and define subcellular compartments …
Studying protein structure and function by native separation–mass spectrometry
G van Schaick, R Haselberg, GW Somsen… - Nature Reviews …, 2022 - nature.com
Alterations in protein structure may have profound effects on biological function. Analytical
techniques that permit characterization of proteins while maintaining their conformational …
techniques that permit characterization of proteins while maintaining their conformational …
Surface-induced dissociation mass spectrometry as a structural biology tool
Native mass spectrometry (nMS) is evolving into a workhorse for structural biology. The
plethora of online and offline preparation, separation, and purification methods as well as …
plethora of online and offline preparation, separation, and purification methods as well as …
A single run, rapid polarity switching method for determination of 30 pharmaceuticals and personal care products in waste water using Q-Exactive Orbitrap high …
The analytical capability of the UPLC-Q Exactive™ Orbitrap MS was exploited for
simultaneous determination of 30 acidic and basic PPCPs in a single run, using rapid …
simultaneous determination of 30 acidic and basic PPCPs in a single run, using rapid …
Collision cross sections of charge-reduced proteins and protein complexes: a database for collision cross section calibration
AQ Stiving, BJ Jones, J Ujma, K Giles… - Analytical …, 2020 - ACS Publications
The use of charge-reducing reagents to generate lower-charge ions has gained popularity
in the field of native mass spectrometry (MS) and ion mobility mass spectrometry (IM-MS) …
in the field of native mass spectrometry (MS) and ion mobility mass spectrometry (IM-MS) …
Charge reduction stabilizes intact membrane protein complexes for mass spectrometry
The study of intact soluble protein assemblies by means of mass spectrometry is providing
invaluable contributions to structural biology and biochemistry. A recent breakthrough has …
invaluable contributions to structural biology and biochemistry. A recent breakthrough has …
Versatile, sensitive, and robust native LC–MS platform for intact mass analysis of protein drugs
Over the past several years, hyphenation of native (nondenaturing) liquid chromatography
(nLC) methods, such as size exclusion chromatography (SEC), ion exchange …
(nLC) methods, such as size exclusion chromatography (SEC), ion exchange …
Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions
Oligomeric proteins assemble with exceptional selectivity, even in the presence of closely
related proteins, to perform their cellular roles. We show that most proteins related by gene …
related proteins, to perform their cellular roles. We show that most proteins related by gene …
Discovery of potent charge-reducing molecules for native ion mobility mass spectrometry studies
There is growing interest in the characterization of protein complexes and their interactions
with ligands using native ion mobility mass spectrometry. A particular challenge, especially …
with ligands using native ion mobility mass spectrometry. A particular challenge, especially …
C-terminal interactions mediate the quaternary dynamics of αB-crystallin
GR Hilton, GKA Hochberg… - … of the Royal …, 2013 - royalsocietypublishing.org
αB-crystallin is a highly dynamic, polydisperse small heat-shock protein that can form
oligomers ranging in mass from 200 to 800 kDa. Here we use a multifaceted mass …
oligomers ranging in mass from 200 to 800 kDa. Here we use a multifaceted mass …