Infectious diseases, along with cancers, are among the main causes of death among
humans worldwide. The production of therapeutic proteins for treating diseases at large …

Much of the biopharmaceutical industry's success over the past 30 years has relied on
products derived from Chinese Hamster Ovary (CHO) cell lines. During this time …

Over twenty-five years ago, perfusion was applied to mammalian cells to produce
therapeutic proteins, mainly sensitive ones. Nowadays, other issues such as process …

A two-step procedure for the design and optimization of mammalian cell perfusion cultures
for therapeutic protein production, for a given expression system, is developed. The …

The biopharmaceutical market is dominated by monoclonal antibodies, the majority of which
are produced in Chinese hamster ovary (CHO) cell lines. Intense cell engineering, in …

Molecular biological methods have emerged as inevitable tools to accompany the process
of cell line development for the generation of stable and highly productive manufacturing cell …

Background Despite technological advances ensuring stable cell culture perfusion
operation over prolonged time, reaching a cellular steady‐state metabolism remains a …

Process intensification aims to increase productivity in biologics manufacturing. Significant
progress has been made in academia, the biopharmaceutical industry, and by the regulatory …

Perfusion processes are gaining higher interest in the industry for the production of
glycoproteins. The intensification that this operation mode can bring is attractive and …

Recent years, intensified fed‐batch culture with ultra‐high seeding density (uHSD‐IFB) is
coming to the forefront of manufacturers' choice for its enhanced productivity. However, the …