Optical chemical imaging seeks to nondestructively acquire spatially resolved chemical
information without the use of labels or probes. The combination of vibrational mid-infrared …

Vibrational spectroscopy and imaging promise molecular information that can be rapidly
acquired without the need for specialized stains or dyes, thereby potentially simplifying and …

Optical microscopy for biomedical samples requires expertise in staining to visualize
structure and composition. Midinfrared (mid-IR) spectroscopic imaging offers label-free …

This paper reports the first use of a novel completely optically based photothermal method
(O-PTIR) for obtaining infrared spectra of both fixed and living cells using a quantum …

Vibrational circular dichroism (VCD) spectroscopy has emerged as a powerful platform to
quantify chirality, a vital biological property that performs a pivotal role in the metabolism of …

Dyes such as hematoxylin and eosin (H&E) and immunohistochemical stains have been
increasingly used to visualize tissue composition in research and clinical practice. We …

Mid-infrared photothermal (MIP) microscopy has been a promising label-free chemical
imaging technique for functional characterization of specimens owing to its enhanced spatial …

Fourier transform infrared (FT-IR) spectroscopic imaging has been widely tested as a tool for
stainless digital histology of biomedical specimens, including for the identification of …

Advancement of discrete frequency infrared (DFIR) spectroscopic microscopes in image
quality and data throughput are critical to their use for analytical measurements. Here, we …

Extended multiplicative signal correction (EMSC) is a widely used preprocessing technique
in infrared spectroscopy. EMSC is a model-based method favored for its flexibility and …