In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

A detailed knowledge of the complex processes that make cells and organisms alive is
fundamental in order to understand diseases and to develop novel drugs and therapeutic …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

Classical structural biology approaches allow structural characterization of biological
macromolecules in vitro, far from their physiological context. Nowadays, thanks to the wealth …

In-cell NMR spectroscopy is a unique tool for characterizing biological macromolecules in
their physiological environment at atomic resolution. Recent progress in NMR instruments …

Conspectus Cellular structural biology methods are needed to characterize biological
processes at atomic resolution in the physiological environment of the cell. Toward this goal …

Understanding biological processes at the molecular level is a pillar of modern science, and
unique insight can be gained by studying living organisms in real time. As a non-destructive …

In-cell nuclear magnetic resonance (NMR) is a method to provide the structural information
of a target at an atomic level under physiological conditions and a full view of the …

In-cell nuclear magnetic resonance spectroscopy (NMR) is a powerful tool to determine the
properties of proteins and nucleic acids within living cells. In-cell NMR can give site-specific …

In-cell NMR spectroscopy was used to screen for drugs that disrupt the interaction between
prokaryotic ubiquitin like protein, Pup, and mycobacterial proteasome ATPase, Mpa. This …