Transposons are mobile genetic elements evolved to execute highly efficient integration of
their genes into the genomes of their host cells. These natural DNA transfer vehicles have …

The widespread clinical implementation of gene therapy requires the ability to stably
integrate genetic information through gene transfer vectors in a safe, effective, and …

Large serine recombinases (LSRs) are DNA integrases that facilitate the site-specific
integration of mobile genetic elements into bacterial genomes. Only a few LSRs, such as …

Gene therapy protocols require robust and long-term gene expression. For two decades,
retrovirus family vectors have offered several attractive properties as stable gene-delivery …

Molecular medicine has entered a high-tech age that provides curative treatments of
complex genetic diseases through genetically engineered cellular medicinal products. Their …

The inherent risks associated with vector insertion in gene therapy need to be carefully
assessed. We analyzed the genome-wide distributions of Sleeping Beauty (SB) and …

Transposons derived from Sleeping Beauty (SB), piggyBac (PB), or Tol2 typically require
cotransfection of transposon DNA with a transposase either as an expression plasmid or …

An ideal tool for gene therapy would enable efficient gene integration at predetermined sites
in the human genome. Here we demonstrate biased genome-wide integration of the …

Future therapeutic use of engineered site-directed nucleases, like zinc-finger nucleases
(ZFNs) and transcription activator-like effector nucleases (TALENs), relies on safe and …

Genetic tools and mutagenesis strategies based on transposable elements are currently
under development with a vision to link primary DNA sequence information to gene …