The efficient large scale production of recombinant proteins depends on the careful
conditioning of the protein as it is isolated and purified to homogeneity. Low protein stability …

The catalytic domain of kinases shows a high degree of sequence homology, especially for
kinases that belong to the same family. They share a common ATP binding site with a …

For years, the use of polyhistidine tags (His-tags) has been a staple in the isolation of
recombinant proteins in immobilized metal affinity chromatography experiments. Their …

In living organisms, genes encoding proteins that contain flavins as a prosthetic group
constitute approximately 2–3% of the total. The fluorescence of flavin cofactors in these …

Differential scanning fluorimetry (DSF) is successfully used as a high-throughput screening
method for the analysis of the protein melting temperature (T m) in the development of …

The Horizon2020 Virus-X project was established in 2015 to explore the virosphere of
selected extreme biotopes and discover novel viral proteins. To evaluate the potential …

Neisseria meningitidis is the causative agent of meningococcal meningitis and sepsis and
remains a significant public health problem in many countries. Efforts to develop a …

In the past decade, thermal melt/thermal shift assays have become a common tool for
identifying ligands and other factors that stabilize specific proteins. Increased stability is …

The beta-proteobacterium Cupriavidus metallidurans is able to grow in metal-contaminated
environments due to having sophisticated metal efflux systems. Here, the contribution of all …

My personal introduction to the environmental outcome from microbial metabolism of
minerals came when I was an undergraduate student in the laboratory of my science advisor …