Transition metals are both essential to enzymatic catalysis and limited in environmental
availability. These two biological facts have together driven organisms to evolve …

We propose experimental strategies to expand our understanding of the role of Ni in plants,
beyond the Ni-metallocenter of urease, still the only identified Ni-containing plant enzyme …

Metalloenzymes often require elaborate metallocenter assembly systems to create
functional active sites. The medically important dinuclear nickel enzyme urease provides an …

Urease is a metalloenzyme essential for the survival of Helicobacter pylori in acidic gastric
environment. Maturation of urease involves carbamylation of Lys219 and insertion of two …

Hydrogenases and ureases play vital metabolic functions in all three domains of life.
However, nickel ions are cytotoxic because they can inactivate enzymes that require less …

Maturation of urease involves post-translational insertion of nickel ions to form an active site
with a carbamylated lysine ligand and is assisted by urease accessory proteins UreD, UreE …

Background Helicobacter pylori is a bacterium strongly associated with gastric cancer. It
thrives in the acidic environment of the gastric niche of large portions of the human …

Helicobacter pylori is a human bacterial pathogen that causes peptic ulcers and has been
designated a Class I carcinogen by the International Agency for Research on Cancer …

Abstract Helicobacter pylori UreF (Hp UreF) is involved in the insertion of Ni 2+ in the urease
active site. The recombinant protein in solution is a dimer characterized by an extensive α …

Colonization of Helicobacter pylori in the acidic environment of the human stomach depends
on the neutralizing activity of urease. Activation of apo-urease involves carboxylation of …