Comparing the effects of additives on protein analysis between desorption electrospray (DESI) and electrospray ionization (ESI)

E Honarvar, AR Venter - Journal of the American Society for Mass …, 2018 - ACS Publications
It is frequently said that DESI-MS follows a similar ionization mechanism as ESI because of
similarities usually observed in their respective mass spectra. However, practical use of …

Charge retention/charge depletion in ESI-MS: experimental evidence

M Thinius, C Polaczek, M Langner… - Journal of the …, 2020 - ACS Publications
The effects of liquid and gas phase additives (chemical modifiers) on the ion signal
distribution for Substance P (SP), recorded with a nanoelectrospray setup, are evaluated …

Stabilization of Labile Lysozyme–Ligand Interactions in Native Electrospray Ionization Mass Spectrometry

Y Du, F Zhao, J Xing, Z Liu, M Cui - Journal of the American …, 2023 - ACS Publications
Flavonoids are polyphenolic secondary metabolites with extensive biological activities and
pharmacological effects. Exploring the interactions of flavonoids with proteins may be helpful …

Charge retention/charge depletion in ESI-MS: theoretical rationale

A Haack, C Polaczek, M Tsolakis… - Journal of the …, 2020 - ACS Publications
Gas phase modification in ESI-MS can significantly alter the charge state distribution of small
peptides and proteins. The preceding paper presented a systematic experimental study on …

The addition of polar organic solvent vapors during the analysis of proteins by DESI-MS

R Javanshad, TL Maser, E Honarvar… - Journal of The …, 2019 - ACS Publications
Exposure of electrospray droplets to organic vapors was shown to dramatically reduce alkali-
metal adduction on protein ions and shift protein charge states. Since DESI-MS is affected …

Stabilizing protein-ligand complexes in ESI–MS using solution additives: Comparing the effects of amino acids and imidazole

EN Kitova, Y Yao, JS Klassen - International Journal of Mass Spectrometry, 2017 - Elsevier
The results of a comparative study of the stabilizing effects of amino acids and imidazole on
electrospray ionization mass spectrometry (ESI–MS) measurements of protein-ligand …

Enhancing signal and mitigating up-front peptide fragmentation using controlled clustering by gas-phase modifiers

B Seale, BB Schneider, JCY Le Blanc - Analytical and bioanalytical …, 2019 - Springer
Up-front CID fragmentation is a phenomenon where molecular ions are activated and
fragment as they enter the atmosphere-to-vacuum region of the mass spectrometer, and …

[PDF][PDF] Kinetic energy measurements for ion dynamics studies in API-MS and Modeling of an FT-QIT

M Thinius - 2021 - scholar.archive.org
This work consists of two parts that are concerned with different aspects of the ion dynamics
in quadrupole ion trap (QIT) mass spectrometers. The first part describes the development …

Fundamentals and Applications of Enhanced fluidity Liquids for Intact Protein Mass Spectrometry Analysis

O Sylvester - 2022 - search.proquest.com
The growing need for fast separations, improved signal sensitivity and selectivity, in the
analysis of intact proteins and non-covalent complexes in the gas-phase has driven the …

[图书][B] Mechanisms and Applications of Improved Protein Analysis by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS)

R Javanshad - 2021 - search.proquest.com
Electrospray ionization mass spectrometry (ESI-MS) is a soft ionization technique that allows
detection of macromolecules, such as intact proteins, by the formation of multiply charged …