Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration

Z Kocsisova, V Coneva - Frontiers in Genome Editing, 2023 - frontiersin.org
Increased understanding of plant genetics and the development of powerful and easier-to-
use gene editing tools over the past century have revolutionized humankind's ability to …

Organelle-targeted gene delivery in plants by nanomaterials

SSY Law, T Miyamoto, K Numata - Chemical Communications, 2023 - pubs.rsc.org
Genetic engineering of plants has revolutionized agriculture and has had a significant
impact on our everyday life. It has allowed for the production of crops with longer shelf lives …

Polymeric Nanocarriers Autonomously Cross the Plant Cell Wall and Enable Protein Delivery for Stress Sensing

Y Zhang, Y Cao, W Jiang, Q Ma, J Shin… - Advanced …, 2024 - Wiley Online Library
Delivery of proteins in plant cells can facilitate the design of desired functions by modulation
of biological processes and plant traits but is currently limited by narrow host range, tissue …

Rational nanoparticle design for efficient biomolecule delivery in plant genetic engineering

Y Zhao, C Thenarianto, C Sevencan, S Rajappa… - Nanoscale, 2024 - pubs.rsc.org
The pressing issue of food security amid climate change necessitates innovative agricultural
practices, including advanced plant genetic engineering techniques. Efficient delivery of …

Relaxation of the Plant Cell Wall Barrier via Zwitterionic Liquid Pretreatment for Micelle‐Complex‐Mediated DNA Delivery to Specific Plant Organelles

T Miyamoto, K Tsuchiya, K Toyooka, Y Goto… - Angewandte …, 2022 - Wiley Online Library
Targeted delivery of genes to specific plant organelles is a key challenge for fundamental
plant science, plant bioengineering, and agronomic applications. Nanoscale carriers have …

Advancing Biomolecule Delivery in Plants: Harnessing Synthetic Nanocarriers to Overcome Multiscale Barriers for Cutting-Edge Plant Bioengineering

T Miyamoto, K Numata - Bulletin of the Chemical Society of …, 2023 - academic.oup.com
The delivery of functional biomolecules, including DNA, RNA, and proteins, into plants
presents a unique opportunity to manipulate plant traits and address challenges related to …

Oligosarcosine conjugation of arginine-rich peptides improves the intracellular delivery of peptide/pDNA complexes

H Yokoo, A Dirisala, S Uchida… - ACS Biomaterials Science …, 2023 - ACS Publications
Cell-penetrating peptides (CPPs), for example, arginine (Arg) rich peptides, are used for the
intracellular delivery of nucleic acids. In this study, oligosarcosine–conjugated Arg-rich …

Nuclear delivery of exogenous gene in mature plants using nuclear location signal and cell-penetrating peptide nanocomplex

J Li, S Li, M Du, Z Song, H Han - ACS Applied Nano Materials, 2022 - ACS Publications
Delivery and expression of exogenous plasmid DNA (pDNA) into mature plants for plant
genetic engineering mainly rely on Agrobacterium-mediated transformation and biolistic …

Peptide-mediated gene and protein delivery systems to plant mitochondria for modifying mitochondrial functions

N Abe, K Numata - Polymer Journal, 2024 - nature.com
Plant mitochondria are essential for energy production and male sterility. The genetic
transformation of plant mitochondria has attracted attention due to its potential to improve the …

A Chimeric Peptide for Shielding Plant Photosynthetic Systems against Excess Light Stress via Chloroplast-Targeted ROS Quenching

T Miyamoto, SR Morey-Yagi, K Numata - JACS Au, 2024 - ACS Publications
The ability to quench reactive oxygen species (ROS) overproduced in plant chloroplasts
under light stress conditions is essential for securing plant photosynthetic performance and …