Recent advances in overexpression of functional recombinant lipases
Heterologous functional expression of the recombinant lipases is typically a bottleneck due
to the expression in the insoluble fraction as inclusion bodies (IBs) which are in inactive …
to the expression in the insoluble fraction as inclusion bodies (IBs) which are in inactive …
Characterize direct protein interactions with enrichable, cleavable and latent bioreactive unnatural amino acids
DD Liu, W Ding, JT Cheng, Q Wei, Y Lin, TY Zhu… - Nature …, 2024 - nature.com
Latent bioreactive unnatural amino acids (Uaas) have been widely used in the development
of covalent drugs and identification of protein interactors, such as proteins, DNA, RNA and …
of covalent drugs and identification of protein interactors, such as proteins, DNA, RNA and …
Catalytically self‐sufficient CYP116B5: Domain switch for improved peroxygenase activity
D Correddu, G Catucci, D Giuriato… - Biotechnology …, 2023 - Wiley Online Library
Self‐sufficient cytochromes P450 of the sub‐family CYP116B have gained great attention in
biotechnology due to their ability to catalyze challenging reactions toward a wide range of …
biotechnology due to their ability to catalyze challenging reactions toward a wide range of …
Drug development for target ribosomal protein rpL35/uL29 for repair of LAMB3R635X in rare skin disease epidermolysis bullosa
A Rathner, P Rathner, A Friedrich, M Wießner… - Skin Pharmacology and …, 2021 - karger.com
Introduction: Epidermolysis bullosa (EB) describes a family of rare genetic blistering skin
disorders. Various subtypes are clinically and genetically heterogeneous, and a lethal …
disorders. Various subtypes are clinically and genetically heterogeneous, and a lethal …
Development and Evaluation of an Immunoinformatics-Based Multi-Peptide Vaccine against Acinetobacter baumannii Infection
Multi-drug-resistant (MDR) Acinetobacter baumannii is an opportunistic pathogen
associated with hospital-acquired infections. Due to its environmental persistence, virulence …
associated with hospital-acquired infections. Due to its environmental persistence, virulence …
Production and Purification of Cysteine-Rich Leptospiral Virulence-Modifying Proteins with or Without mCherry Fusion
R Chaurasia, C Liang, K How, DS Vieira, JM Vinetz - The Protein Journal, 2023 - Springer
Recombinant fluorescent fusion proteins are fundamental to advancing many aspects of
protein science. Such proteins are typically used to enable the visualization of functional …
protein science. Such proteins are typically used to enable the visualization of functional …
Evaluation of recombinant Babesia gibsoni thrombospondin-related adhesive protein (BgTRAP) for the sero-diagnosis of canine babesiosis
CK Deepa, A Varghese, KGA Kumar, A Nandini… - Experimental …, 2023 - Elsevier
Canine babesiosis, caused by Babesia gibsoni is one of the most significant tick-borne
illnesses across the world. Light microscopy as well as polymerase chain reaction may fail in …
illnesses across the world. Light microscopy as well as polymerase chain reaction may fail in …
SANS investigation of fungal loosenins reveal substrate dependent impacts of protein 1 action on inter-fibril distance and packing order of cellulosic substrates
D Dahiya, Z Péter-Szabó, M Senanayake… - Research …, 2024 - pmc.ncbi.nlm.nih.gov
Background: Microbial expansin-related proteins include fungal loosenins, which have been
previously shown to disrupt cellulose networks and enhance the enzymatic conversion of …
previously shown to disrupt cellulose networks and enhance the enzymatic conversion of …
[HTML][HTML] mCherry Fusion Proteins Facilitate Production of Recombinant, Cysteine-Rich Leptospira interrogans Proteins in Escherichia coli
R Chaurasia, C Liang, K How, DS Vieira… - Research …, 2023 - ncbi.nlm.nih.gov
Background Recombinant fluorescent fusion proteins are fundamental to advancing many
aspects of protein science. Such proteins are typically used to enable the visualization of …
aspects of protein science. Such proteins are typically used to enable the visualization of …
An efficient method for recombinant production of human alpha synuclein in Escherichia coli using thioredoxin as a fusion partner
B Saffari, M Amininasab, S Sheikhi… - Preparative …, 2020 - Taylor & Francis
Herein, we describe a simple and efficient approach to produce recombinant human α-
synuclein (hAS) with high purity from Escherichia coli (E. coli). The cDNA for hAS was …
synuclein (hAS) with high purity from Escherichia coli (E. coli). The cDNA for hAS was …