High-throughput single-cell profiling provides an unprecedented ability to uncover the
molecular states of millions of cells. These technologies are, however, destructive to cells …

Comprehensively charting the biologically causal circuits that govern the phenotypic space
of human cells has often been viewed as an insurmountable challenge. However, in the last …

Genome editing has been a transformative force in the life sciences and human medicine,
offering unprecedented opportunities to dissect complex biological processes and treat the …

MicroRNAs (miRNAs) are small non-coding RNAs (18–26 nucleotides) that regulate gene
expression by interacting with target mRNAs, affecting various physiological and …

Programmable genome-engineering technologies, such as CRISPR (clustered regularly
interspaced short palindromic repeats) nucleases and massively parallel CRISPR screens …

Cells are the fundamental units of biological systems and exhibit unique development
trajectories and molecular features. Our exploration of how the genomes orchestrate the …

Alternative transcription start site usage (ATSS) is a widespread regulatory strategy that
enables genes to choose between multiple genomic loci for initiating transcription. This …

Layer 5 extratelencephalic (ET) neurons are present across neocortical areas and send
axons to multiple subcortical targets,,,,–. Two cardinal subtypes exist,:(1) Slco2a1 …

Single-cell CRISPR screens link genetic perturbations to transcriptional states, but high-
throughput methods connecting these induced changes to their regulatory foundations are …

Temporal ordering of cellular events offers fundamental insights into biological phenomena.
Although this is traditionally achieved through continuous direct observations 1, 2, an …