Microarray experiments and factors which affect their reliability

R Jaksik, M Iwanaszko, J Rzeszowska-Wolny… - Biology direct, 2015 - Springer
Oligonucleotide microarrays belong to the basic tools of molecular biology and allow for
simultaneous assessment of the expression level of thousands of genes. Analysis of …

Segregation of spermatozoa within sperm storage tubules of fowl and turkey hens

LM King, JP Brillard, WM Garrett, MR Bakst… - …, 2002 - rep.bioscientifica.com
Sertoli cells and germ cells are separated from the interstitial blood capillaries by an
extracellular matrix and the peritubular cells, which constitute a barrier to the movement of …

Robust transcriptional signatures for low-input RNA samples based on relative expression orderings

H Liu, Y Li, J He, Q Guan, R Chen, H Yan, W Zheng… - BMC genomics, 2017 - Springer
Background It is often difficult to obtain sufficient quantity of RNA molecules for gene
expression profiling under many practical situations. Amplification from low-input samples …

Transcriptome-stable isotope probing provides targeted functional and taxonomic insights into microaerobic pollutant-degrading aquifer microbiota

LM Bradford, G Vestergaard, A Táncsics… - Frontiers in …, 2018 - frontiersin.org
While most studies using RNA-stable isotope probing (SIP) to date have focused on
ribosomal RNA, the detection of 13C-labeled mRNA has rarely been demonstrated. This …

Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling

M Clément-Ziza, D Gentien, S Lyonnet, JP Thiery… - BMC genomics, 2009 - Springer
Background For more than a decade, microarrays have been a powerful and widely used
tool to explore the transcriptome of biological systems. However, the amount of biological …

Efficient derivation of bovine embryonic stem cells needs more than active core pluripotency factors

J Maruotti, M Muñoz, SA Degrelle… - Molecular …, 2012 - Wiley Online Library
Pluripotency can be captured in vitro, providing that the culture environment meets the
requirements that avoid differentiation while stimulating self‐renewal. From studies in the …

Gene expression within the extended amygdala of 5 pairs of rat lines selectively bred for high or low ethanol consumption

WJ McBride, MW Kimpel, JN McClintick, ZM Ding… - Alcohol, 2013 - Elsevier
The objectives of this study were to determine innate differences in gene expression in 2
regions of the extended amygdala between 5 different pairs of lines of male rats selectively …

Energy and lipid metabolism gene expression of D18 embryos in dairy cows is related to dam physiological status

D Valour, SA Degrelle, AA Ponter… - Physiological …, 2014 - journals.physiology.org
We analyzed the change in gene expression related to dam physiological status in day (D)
18 embryos from growing heifers (GH), early lactating cows (ELC), and late lactating cows …

[PDF][PDF] A small set of extra-embryonic genes defines a new landmark for bovine embryo staging

SA Degrelle, KA Lê Cao, Y Heyman, RE Everts… - Reproduction, 2011 - Citeseer
Axis specification in mouse is determined by a sequence of reciprocal interactions between
embryonic and extra-embryonic tissues so that a few extra-embryonic genes appear as …

Uncoupled embryonic and extra-embryonic tissues compromise blastocyst development after somatic cell nuclear transfer

SA Degrelle, F Jaffrezic, E Campion, KA Le Cao… - PloS one, 2012 - journals.plos.org
Somatic cell nuclear transfer (SCNT) is the most efficient cell reprogramming technique
available, especially when working with bovine species. Although SCNT blastocysts …