Specimen preparation for high-resolution cryo-EM
LA Passmore, CJ Russo - Methods in enzymology, 2016 - Elsevier
Imaging a material with electrons at near-atomic resolution requires a thin specimen that is
stable in the vacuum of the transmission electron microscope. For biological samples, this …
stable in the vacuum of the transmission electron microscope. For biological samples, this …
Better Cryo-EM specimen preparation: how to deal with the air–water Interface?
N Liu, HW Wang - Journal of Molecular Biology, 2023 - Elsevier
Cryogenic electron microscopy (cryo-EM) is now one of the most powerful and widely used
methods to determine high-resolution structures of macromolecules. A major bottleneck of …
methods to determine high-resolution structures of macromolecules. A major bottleneck of …
[HTML][HTML] Structure of microbial nanowires reveals stacked hemes that transport electrons over micrometers
Summary Long-range (> 10 μm) transport of electrons along networks of Geobacter
sulfurreducens protein filaments, known as microbial nanowires, has been invoked to …
sulfurreducens protein filaments, known as microbial nanowires, has been invoked to …
[HTML][HTML] An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology
Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …
Artificial intelligence in cryo-EM protein particle picking: recent advances and remaining challenges
Cryo-electron microscopy (cryo-EM) has revolutionized structural biology by enabling the
determination of high-resolution 3-Dimensional (3D) structures of large biological …
determination of high-resolution 3-Dimensional (3D) structures of large biological …
Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports
Poor partitioning of macromolecules into the holes of holey carbon support grids frequently
limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here …
limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here …
Recent technical advances in sample preparation for single-particle cryo-EM
Y Xu, S Dang - Frontiers in molecular biosciences, 2022 - frontiersin.org
Cryo-sample preparation is a vital step in the process of obtaining high-resolution structures
of macromolecules by using the single-particle cryo–electron microscopy (cryo-EM) method; …
of macromolecules by using the single-particle cryo–electron microscopy (cryo-EM) method; …
Detergent modulates the conformational equilibrium of SARS-CoV-2 Spike during cryo-EM structural determination
The Spike glycoprotein of SARS-CoV-2 mediates viral entry into the host cell via the
interaction between its receptor binding domain (RBD) and human angiotensin-converting …
interaction between its receptor binding domain (RBD) and human angiotensin-converting …
Comprehensive microcrystal electron diffraction sample preparation for cryo-EM
Microcrystal electron diffraction (MicroED) has advanced structural methods across a range
of sample types, from small molecules to proteins. This cryogenic electron microscopy (cryo …
of sample types, from small molecules to proteins. This cryogenic electron microscopy (cryo …
Structure and membrane topography of the vibrio-type secretin complex from the type 2 secretion system of enteropathogenic Escherichia coli
The β-barrel assembly machinery (BAM) complex is the core machinery for the assembly of
β-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria …
β-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria …