Advances in CRISPR technology have immensely improved our ability to manipulate nucleic
acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further …

The use of Drosophila cell cultures has positively impacted both fundamental and
biomedical research. The most widely used cell lines: Schneider, Kc, the CNS and imaginal …

Genome-wide screens in Drosophila cells have offered numerous insights into gene
function, yet a major limitation has been the inability to stably deliver large multiplexed DNA …

Perfluorooctanesulfonic acid (PFOS) is a persistent synthetic surfactant widely detected in
the environment. Developmental PFOS exposures are associated with low birth weight and …

Insect cells can be used for the efficient production of heterologous proteins. The
baculovirus expression vector system (BEVS) in Spodoptera frugiperda cells and the stable …

The generation of monoclonal cell lines is an important early process development step for
recombinant protein production. Although single-cell cloning is an established method in …

Transfection of transgenes into Drosophila cultured cells is a standard approach for studying
gene function. However, the number of transgenes present in the cell following transient …

The generation of Drosophila stable cell lines has become invaluable for complementing in
vivo experiments and as tools for genetic screens. Recent advances utilizing attP/PhiC31 …

Background Temperature change affects the myriad of concurrent cellular processes in a
non-uniform, disruptive manner. While endothermic organisms minimize the challenge of …

Iron plays a critical role in many biological processes. The ability of iron to gain and lose
electrons allows it to be involved in a wide variety of biochemical reactions. Organismal iron …