Highlights•Camelid nanobodies (or VHHs) facilitate proteins crystallization.•They
preferentially bind concave surfaces such as protein active sites or hinges.•They are easily …

Drug-resistant bacteria pose an increasingly serious threat to mankind all over the world.
However, the currently available clinical treatments do not meet the urgent demand …

Small near-infrared (NIR) fluorescent proteins (FPs) are much needed as protein tags for
imaging applications. We developed a 17 kDa NIR FP, called miRFP670nano3, which …

Nanobodies are the recombinant antigen-recognizing domains of the minimalistic heavy
chain-only antibodies produced by camels and llamas. Nanobodies can be easily …

The last five years have witnessed major developments in the understanding of the allosteric
phenomenon, broadly defined as coupling between remote molecular sites. Such advances …

A substantial fraction of eukaryotic proteins is folded and modified in the endoplasmic
reticulum (ER) prior to export and secretion. Proteins that enter the ER but fail to fold …

Synthetic mRNA (mRNA) enables transgene expression without the necessity of nuclear
import and the risk of insertional mutagenesis, which makes it an attractive tool for medical …

Escherichia coli Dihydrofolate reductase is an important enzyme that is essential for the
survival of the Gram‐negative microorganism. Inhibitors designed against this enzyme have …

We describe three optical tags, ArrayG, ArrayD and ArrayG/N, for intracellular tracking of
single molecules over milliseconds to hours. ArrayG is a fluorogenic tag composed of a …

Allostery commonly refers to the mechanism that regulates protein activity through the
binding of a molecule at a different, usually distal, site from the orthosteric site. The …