Detecting biothreat agents: From current diagnostics to developing sensor technologies

SA Walper, G Lasarte Aragonés, KE Sapsford… - ACS …, 2018 - ACS Publications
Although a fundamental understanding of the pathogenicity of most biothreat agents has
been elucidated and available treatments have increased substantially over the past …

Real-time PCR as a diagnostic tool for bacterial diseases

M Maurin - Expert review of molecular diagnostics, 2012 - Taylor & Francis
In recent years, quantitative real-time PCR tests have been extensively developed in clinical
microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial …

An evaluation of commercial DNA extraction kits for the isolation of bacterial spore DNA from soil

SM Dineen, R Aranda IV, DL Anders… - Journal of applied …, 2010 - academic.oup.com
Aims: To evaluate six commercial DNA extraction kits for their ability to isolate PCR‐quality
DNA from Bacillus spores in various soil samples. Methods and Results: Three soils were …

Fluorescent nanoparticles: Sensing pathogens and toxins in foods and crops

KP Burris, CN Stewart Jr - Trends in food science & technology, 2012 - Elsevier
Emerging tools in molecular nanotechnology will be useful to monitor and quantify pathogen
levels and toxin production inter-and intra-cellularly and on the surface of foods. Recent …

A multiplex real-time PCR for identifying and differentiating B. anthracis virulent types

PR Wielinga, RA Hamidjaja, J Ågren… - International journal of …, 2011 - Elsevier
Bacillus anthracis is closely related to the endospore forming bacteria Bacillus cereus and
Bacillus thuringiensis. For accurate detection of the life threatening pathogen B. anthracis, it …

A competitive fluorescence assay based on free-complementary DNA for ochratoxin A detection

A Hitabatuma, Y Pang, L Yu, X Shen - Food Chemistry, 2021 - Elsevier
An ultrasensitive, rapid, and specific method for Ochratoxin A (OTA) detection was designed
using complementary sequence to aptamer as a target of molecular beacon (MB). The …

Ultrasensitive Detection of Bacillus anthracis by Real-Time PCR Targeting a Polymorphism in Multi-Copy 16S rRNA Genes and Their Transcripts

P Braun, MDT Nguyen, MC Walter, G Grass - International Journal of …, 2021 - mdpi.com
The anthrax pathogen Bacillus anthracis poses a significant threat to human health.
Identification of B. anthracis is challenging because of the bacterium's close genetic …

Identification of a Bacillus anthracis specific indel in the yeaC gene and development of a rapid pyrosequencing assay for distinguishing B. anthracis from the B …

NZ Ahmod, RS Gupta, HN Shah - Journal of microbiological methods, 2011 - Elsevier
Bacillus anthracis, the causative agent of anthrax, is a potential source of bioterrorism. The
existing assays for its identification lack specificity due to the close genetic relationship it …

The response and factors of microbial aerosol emission from the sludge bio-drying process

Y Wang, L Li, J Ma, Y Han - Waste Management, 2024 - Elsevier
Exposure to high levels of microbial contaminants during waste disposal leads to the
development of various diseases, including respiratory symptoms and gastrointestinal …

[HTML][HTML] Molecular beacons in diagnostics

S Tyagi, FR Kramer - F1000 medicine reports, 2012 - ncbi.nlm.nih.gov
Recent technical advances have begun to realize the potential of molecular beacons to test
for diverse infections in clinical diagnostic laboratories. These include the ability to test for …