In 1925, 5-methylcytosine was first reported in bacteria. However, its biological importance
was not intuitive for several decades. After this initial lag, the ubiquitous presence of this …

RNA-based gene therapy requires therapeutic RNA to function inside target cells without
eliciting unwanted immune responses. RNA can be ferried into cells using non-viral drug …

Cell-based therapeutics are an emerging modality with the potential to treat many currently
intractable diseases through uniquely powerful modes of action. Despite notable recent …

Epigenetics regulates gene expression and has been confirmed to play a critical role in a
variety of metabolic diseases, such as diabetes, obesity, non-alcoholic fatty liver disease …

The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …

The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …

Gene therapy is at an inflection point. Recent successes in genetic medicine have paved the
path for a broader second wave of therapies and laid the foundation for next-generation …

The recent maturation of single-cell RNA sequencing (scRNA-seq) technologies has
coincided with transformative new methods to profile genetic, epigenetic, spatial, proteomic …

Adipose tissues are dynamic tissues that play crucial physiological roles in maintaining
health and homeostasis. Although white adipose tissue and brown adipose tissue are …

Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …