The DNA methylation field has matured from a phase of discovery and genomic
characterization to one seeking deeper functional understanding of how this modification …

In mammals, DNA methylation in the form of 5-methylcytosine (5mC) can be actively
reversed to unmodified cytosine (C) through TET dioxygenase-mediated oxidation of 5mC to …

Bisulfite sequencing detects 5mC and 5hmC at single-base resolution. However, bisulfite
treatment damages DNA, which results in fragmentation, DNA loss, and biased sequencing …

Abstract In mammals, DNA 5-hydroxymethylcytosine (5hmC) is involved in methylation
reprogramming during early embryonic development. Yet, to what extent 5hmC participates …

The development of animal embryos is initially directed by maternal gene products. Then,
during the maternal-to-zygotic transition (MZT), developmental control is handed to the …

A remarkable epigenetic remodelling process occurs shortly after fertilization, which restores
totipotency to the zygote. This involves global DNA demethylation, chromatin remodelling …

The differences between individual cells can have profound functional consequences, in
both unicellular and multicellular organisms. Recently developed single-cell mRNA …

Recent technological advances have enabled DNA methylation to be assayed at single-cell
resolution. However, current protocols are limited by incomplete CpG coverage and hence …

BACKGROUND It has long been thought that the factors affecting embryo and foetal
development were exclusively maternally derived; hence, if issues regarding fertility and …

Single-cell multi-omics has recently emerged as a powerful technology by which different
layers of genomic output—and hence cell identity and function—can be recorded …